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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Methods. 2013 Oct 1;65(1):139–147. doi: 10.1016/j.ymeth.2013.09.015

Figure 4.

Figure 4

Random vs. site-specific conjugation of a chelating agent to an ALCAM-specific cys-diabody. Conjugation to random lysine residues was accomplished using NHS-DOTA; site-specific conjugation was conducted by mild reduction using TCEP followed by reaction with maleimido-DOTA. On the right, mass spectrometry analysis reveals a range of modification (4–8) by the random method, and uniform addition of a single DOTA moiety to each monomer chain of the cys-diabody following site-specific conjugation (K. McCabe, R. Moore., T. Lee, unpublished).