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Time course of the tryptic digest of (A) naïve JKC and (B) autophosphorylated pJKC. Proteins were incubated at room temperature for the indicated times with a 1:1000 wt:wt ratio of enzyme to substrate followed by SDS-PAGE analysis. Arrow indicates the mobility of the undigested protein and MW is the lane with molecular mass markers of 46 kDa and 30 kDa.
