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RifDH10-catalyzed dehydration. a) syn-Dehydration of a (2S,3S)-2-methyl-3-hydroxyacyl thioester substrate generates a trans (E) double bond. b) Stereodiagram showing (2S,3S)-2-methyl-3-hydroxyacyl thioester substrate modeled into the active site of RifDH10. See Figure S2 for modeling of diastereomeric thioesters into the active site.
