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. Author manuscript; available in PMC: 2014 Mar 13.
Published in final edited form as: Oncogene. 2011 May 23;30(45):4590–4600. doi: 10.1038/onc.2011.166

Figure 3.

Figure 3

Reduction of endogenous RasGRP3 expression inhibited anchorage independent growth and rate of tumor growth of both M14 and SK-MEL-5 cell lines. (A and B) Colonies were imaged 10 days after plating the cells in soft agar. The number of colonies larger than the median size of the corresponding control (colonies of the cells expressing the scrambled control shRNA 1 in the absence of tetracycline) were counted and expressed as a percent of the colony number of the control. Values represent mean +/− SEM of the three independent experiments. (C and D) NOD.SCID/NCr mice were injected subcutaneously in the flanks with 1×107 cells/injection of the indicated M14 and SK-MEL-5 derived lines. On the eighth day after injection, half the animals in each treatment group were shifted to food containing deoxytetracycline. The animals were sacrificed 12 weeks (M14) or 8 weeks (SK-MEL-5) after injection, and the tumors were excised and weighed.