AIM-100 inhibits pTyr267-AR binding to AREs. A,C,E: Serum and androgen-depleted LAPC4 cells were treated (or untreated) with DHT (5 nM, 16 hr) or EGF (10 ng/ml, 1 hr), and AIM-100 (0.8 μM, 16 hr) and ChIP analysis for total AR binding to the PSA(A), TMPRSS2 (C), and NKX3.1 (E) AREs was performed followed by quantitative PCR. Data shown are representative of three independent experiments. B,D,F: Serum and androgen-depleted LAPC4 cells were treated with DHT (5 nM, 16 hr) or EGF (10 ng/ml, 1 hr), and AIM-100 (0.8 μM, 16 hr) and ChIP analysis for pTyr267-AR binding to the PSA (B), TMPRSS2 (D) and NKX3.1 (F) AREs was performed followed by quantitative PCR. Data shown are representative of three independent experiments. *P≤ 0.001; **P≤ 0.006; ***P≤0.014.