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. 1994 Dec 1;13(23):5517–5522. doi: 10.1002/j.1460-2075.1994.tb06888.x

Protein splicing: an analysis of the branched intermediate and its resolution by succinimide formation.

M Q Xu 1, D G Comb 1, H Paulus 1, C J Noren 1, Y Shao 1, F B Perler 1
PMCID: PMC395513  PMID: 7988548

Abstract

Protein splicing involves the excision of an internal domain from a precursor protein and the ligation of the external domains so as to generate two new proteins. Study of this process has recently been facilitated by the isolation of a precursor and a branched intermediate from a thermophilic protein splicing element expressed in a foreign protein context. Two aspects of protein splicing are examined in this paper. We demonstrate a succinimide at the C-terminus of the spliced internal protein, implicating cyclization of asparagine in resolution of the branched intermediate, and we identify an alkali-labile bond in the branched intermediate. A revised protein splicing model based on these experimental results is presented.

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Selected References

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