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. 2013 Jan 22;8(1):123–127. doi: 10.1007/s12104-013-9466-6

Backbone and partial side chain assignment of the microtubule binding domain of the MAP1B light chain

Zsuzsanna Orbán-Németh 1, Morkos A Henen 2, Leonhard Geist 2, Szymon Żerko 3, Saurabh Saxena 3, Jan Stanek 3, Wiktor Koźmiński 3, Friedrich Propst 1,✉, Robert Konrat 2,✉
PMCID: PMC3955483  PMID: 23339032

Abstract

Microtubule-associated protein 1B (MAP1B) is a classical high molecular mass microtubule-associated protein expressed at high levels in the brain. It confers specific properties to neuronal microtubules and is essential for neuronal differentiation, brain development and synapse maturation. Misexpression of the protein contributes to the development of brain disorders in humans. However, despite numerous reports demonstrating the importance of MAP1B in regulation of the neuronal cytoskeleton during neurite extension and axon guidance, its mechanism of action is still elusive. Here we focus on the intrinsically disordered microtubule binding domain of the light chain of MAP1B. In order to obtain more detailed structural information about this domain we assigned NMR chemical shifts of backbone and aliphatic side chain atoms.

Keywords: Microtubule-associated protein, Microtubule binding domain, Random sampling, Intrinsically disordered protein

Biological context

During development and repair of the nervous system, individual neurons extend long processes to connect to each other to build or restore a network capable of information processing. Elaboration of these processes, axons and dendrites, crucially depends on microtubules, one component of the neuronal cytoskeleton. The dynamic behavior, the stability and the properties of microtubules are temporarily and spatially regulated by a plethora of microtubule-associated proteins (MAPs). Among these are the 3 members of the MAP1 family of proteins, MAP1A, MAP1B and MAP1S. All three are protein complexes consisting of heavy and light chains and share isolated domains of sequence homology (Schoenfeld et al. 1989; Orban-Nemeth et al. 2005).

The best characterized of these proteins is MAP1B, a large neuronal protein essential for neuronal network formation during murine brain development (Edelmann et al. 1996; Takei et al. 1997; Gonzalez-Billault et al. 2000; Meixner et al. 2000) and for the maturation of synapses (Tortosa et al. 2011). Overexpression or deregulation of MAP1B activity have been associated with fragile X mental retardation (Brown et al. 2001; Zhang et al. 2001), giant axonal neuropathy (Allen et al. 2005), and ataxia (Opal et al. 2003) in humans.

The MAP1B protein complex consists of a 300-kDa heavy chain and at least one light chain termed LC1. The light chain is around 250 amino acids (32 kDa) in size and has several important functional domains. Via its NH2-terminal domain it is capable of interacting with and changing the conformation of microtubules (Tögel et al. 1998). The light chains of MAP1A and MAP1S contain a functionally related microtubule binding domain in their respective NH2 termini; however, there is no sequence homology. In contrast, the COOH-terminal half of the MAP1B light chain is conserved in function as well as sequence among all MAP1 proteins. Via this part of the molecule the light chains can interact with actin filaments and with the heavy chains for the formation of the respective MAP1 protein complex (Tögel et al. 1998; Noiges et al. 2002, 2006; Orban-Nemeth et al. 2005). The interaction of the MAP1B light chain with microtubules can be regulated by posttranslational modification, in particular S-nitrosylation, at an identified cysteine residue in its COOH terminus (Stroissnigg et al. 2007). Being able to bind to microtubules as well as F-actin, MAP1B is considered to be a coupling protein between the two components of the neuronal cytoskeleton (Bouquet et al. 2007). Such proteins are postulated to be essential for orchestrated regulation of the cytoskeleton during neuronal differentiation (Lowery and Van Vactor 2009). However, despite the fact that MAP1B has been discovered many years ago and despite its demonstrated involvement in human disease a clear understanding of its function is still missing.

Meta structure analysis of the MAP1B light chain suggests that the positively charged NH2-terminal microtubule binding domain is intrinsically disordered, whereas the conserved COOH terminus is predicted to be structured. To obtain further insight into how this protein can modulate microtubule properties we initiated NMR structure determination of the NH2-terminal microtubule binding domain. This analysis might eventually also shed light on specific functions of microtubules during neuronal differentiation. Here we report near complete backbone assignment as well as partial assignment of aliphatic side chain atoms, using sophisticated 5D triple resonance NMR experiments.

Methods and experiments

Expression and purification of the NH2 terminus of the MAP1B light chain

A cDNA fragment encoding the NH2-terminal domain of the rat MAP1B light chain (amino acids 2212–2338, NP_062090.1) was cloned into a pET-15b (Novagen) derived expression vector. The resulting plasmid, pMA25His, encodes the microtubule binding domain of the MAP1B light chain fused to an NH2-terminal tag containing 6 histidines (MGSSHHHHHHSSGLVPRGSHMEF). The sequence was confirmed to be correct. pMA25His plasmid was introduced into BL21Codon Plus(DE3) cells (Stratagene) by heat shock at 42 °C for 90 s. Cells were grown overnight in LB medium at 37 °C supplemented with 50 μg/ml ampicillin and 25 μg/ml chloramphenicol. This culture was diluted 1:40 in 2 l LB with antibiotics and was incubated at 37 °C until the culture reached an OD600 of 0.7. Cells were collected at 5,000 rpm for 12 min and resuspended in half the volume of modified M9 minimal medium (Marley et al. 2001) supplemented with 1 g of 15NH4Cl and 2 g of D-[13C]glucose (Cambridge Isotope Laboratories) and trace elements instead of basal vitamins. Cells were incubated for one additional hour at 37 °C. Recombinant protein synthesis was induced by the addition of isopropyl-β-d-thiogalactopyranoside (IPTG) to a final concentration of 0.5 mM, and incubation was continued overnight at 18 °C. The cells were collected by centrifugation at 4,000 rpm for 15 min and resuspended in 10 ml of ice-cold lysis buffer (50 mM NaH2PO4, 300 mM NaCl, pH 8). Cells were lysed by sonication at 80 % for 20 × 10 s in 10 ml lysis buffer containing 40 mg lysozyme, 1 mg DNase I, and 4 EDTA free complete mini protease inhibitor tablets (Roche). The lysate was cleared by centrifugation at 13,000 rpm for 30 min. The supernatant was loaded onto an FPLC Ni2+-loaded HisTrap FF crude 5 ml affinity column (GE Healthcare). The column was washed with lysis buffer containing 20 mM imidazole. The recombinant protein was eluted with lysis buffer containing 75 mM imidazole, concentrated approximately 10-fold by centrifugation through an Amicon Ultra-15 centrifugal 3 K filter device and loaded onto a Superdex 200 HiLoad 16/60 prep grade gel filtration column (GE Healthcare) equilibrated in lysis buffer. The final yield of homogenous recombinant protein was approximately 2 mg/l of bacterial culture. For NMR analysis, protein samples were concentrated to at least 700 μM and pH was changed to 5.

NMR experiments

All spectra were acquired at 298 K on an Agilent Direct Drive 700 MHz spectrometer using the standard 5 mm 1H–13C–15N triple-resonance probehead.

The backbone 1H, 13C and 15N resonances were assigned using sparse random sampling of indirectly detected time domains, in order to increase resolution. A 3D HNCO experiment was used as a base spectrum for SMFT (Sparse Multidimensional Fourier Transform) processing of higher dimensionality experiments (Kazimierczuk et al. 2009). Backbone assignment was achieved using 5D HN(CA)CONH (Kazimierczuk et al. 2010), (HACA)CON(CA)CONH (Zawadzka-Kazimierczuk et al. 2012b), (H)NCO(NCA)CONH (Zawadzka-Kazimierczuk et al. 2012b) and 4D HNCACO (Zawadzka-Kazimierczuk et al. 2010) experiments. Side-chain assignments were obtained using 5D HabCabCONH (Kazimierczuk et al. 2010), and H(CC-tocsy)CONH (Kazimierczuk et al. 2009) experiments.

All NMR data sets were processed by multidimensional Fourier transformation using the home written software package (http://nmr700.chem.uw.edu.pl/formularz.html). The resonance assignment was performed using the TSAR program (Zawadzka-Kazimierczuk et al. 2012a). The input data for TSAR was prepared and analyzed using the Sparky software (Goddard and Kneller 2008). Table 1 shows the maximum evolution times and spectral width used for the acquisition of the spectra.

Table 1.

Maximum evolution times (tmax, ms) and spectral width (sw, kHz) used for acquisition of spectra for the NH2 terminus of the light chain of MAP1B

3D
HNCO		 4D
HNCACO		 5D HabCabCONH 5D HN(CA)CONH 5D
H(CC-tocsy)CONH		 5D
(H)NCO(NCA)CONH		 5D
(HACA)CON(CA)CONH
Number of points 750 1800 715 780 1285 700 900
Experiment duration (h) 5 23 18 20 42 18 19
sw1 2.8 2.8 4 6 8 2.5 3.8
sw2 2.5 6.2 14 2.5 18 2.8 2.8
sw3 2.5 2.8 2.8 2.8 2.8 2.8
sw4 2.5 2.5 2.5 2.5 2.5
t max1 100 50 15 20 15 30 30
t max2 100 10 7 50 8 30 30
t max3 75 30 50 30 30 30
t max4 50 50 50 50 50
Sampling density versus conventional 1.1 × 10−2 1.1 × 10−3 1.2 × 10−5 3.0 × 10−6 7.1 × 10−6 1.1 × 10−5 9.0 × 10−6
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Assignments and data deposition

The 1H–15N HSQC spectrum of the NH2 terminus of the light chain of MAP1B shows the for intrinsically disordered proteins typical narrow peak dispersion in the 1H dimension (Fig. 1). The use of the aforementioned 5D experiments allowed us to nearly completely assign backbone atoms by resolving extensively overlapping signals in conventional 2D and 3D spectra. 89 % of backbone 15N, 95.8 % of 1HN, 88.2 % of 13Cα, 88.2 % of 1Hα and 88.2 % of 13CO resonances could be assigned (calculated without His-tag). Additionally, HabCabCONH and H(CC-tocsy)CONH spectra allowed the assignment of several aliphatic side chain atoms. 87.4 % of 13Cβ, 87.4 % of 1Hβ, 79.2 % of 13Cγ, 85.2 % of 1Hγ, 51.6 % 13Cδ and 62.7 % of 1Hδ could be assigned. For 7 out of 29 lysine side chains we also obtained the 13Cε and 1Hε assignments. Figure 2 outlines sequential resonance assignment in a 5D (H)NCO(NCA)CONH and HN(CA)CONH experiment, showing strips of sequential residues. Secondary chemical shifts for 13Cα (Fig. 3) show only minor deviations from random coil chemical shift values with slight α-helical propensities at the NH2 terminus, corroborating the finding of the Meta Structure analysis (not shown).

Fig. 1.

Fig. 1

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1H–15N HSQC spectrum of the NH2 terminus of the light chain of MAP1B at pH5 and 298 K. Assignments of backbone amides are labeled in single letter amino acid code and residue number (His6-tag: 1–23; NH2 terminus of the light chain of MAP1B: 24–150)

Fig. 2.

Fig. 2

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2D spectral planes for consecutive amino acids in the NH2 terminus of the light chain of MAP1B obtained by SMFT processing of the 5D randomly sampled signal. 2D cross-sections of a 5D (H)NCO(NCA)CONH (Ni–COi−1 and Ni−1–COi−2) and b 5D HN(CA)CONH (HNi–Ni and HNi+1–Ni+1)

Fig. 3.

Fig. 3

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Graph showing 13Cα secondary chemical shifts of the NH2 terminus of the light chain of MAP1B. Random coil chemical shift values were obtained using the neighborhood-corrected IDP chemical shift library (Tamiola et al. 2010)

The 1H, 13C and 15N chemical shift assignments have been deposited in the BioMagResBank database (http://www.bmrb.wisc.edu) under the accession number 18895.

Acknowledgments

NMR experiments were carried out in the Structural Research Laboratory at the Faculty of Chemistry, University of Warsaw. This work was supported by the Bio-NMR Project No. 261863 funded by European Commissions Framework Program 7 (FP7). S.S., S.Ż. and W.K. thank the Foundation for Polish Science for the support with the TEAM Programme, co-financed by the European Union within the European Regional Development Fund. This research was also supported by grant P20839-B09 (to F.P.) from the Austrian Science Fund (FWF) and ÖAD (Ku with Egypt, art 9/2) (to M.A.H).

Contributor Information

Friedrich Propst, Phone: +43-1-427752858, FAX: +43-1-427752854, Email: friedrich.propst@univie.ac.at.

Robert Konrat, Phone: +43-1-427752202, FAX: +43-1-42779522, Email: robert.konrat@univie.ac.at.

References

  1. Allen E, Ding J, Wang W, Pramanik S, Chou J, Yau V, Yang Y. Gigaxonin-controlled degradation of MAP1B light chain is critical to neuronal survival. Nature. 2005;438(7065):224–228. doi: 10.1038/nature04256. [DOI] [PubMed] [Google Scholar]
  2. Bouquet C, Ravaille-Veron M, Propst F, Nothias F. MAP1B coordinates microtubule and actin filament remodeling in adult mouse Schwann cell tips and DRG neuron growth cones. Mol Cell Neurosci. 2007;36(2):235–247. doi: 10.1016/j.mcn.2007.07.002. [DOI] [PubMed] [Google Scholar]
  3. Brown V, Jin P, Ceman S, Darnell JC, O’Donnell WT, Tenenbaum SA, Jin X, Feng Y, Wilkinson KD, Keene JD, Darnell RB, Warren ST. Microarray identification of FMRP-associated brain mRNAs and altered mRNA translational profiles in fragile X syndrome. Cell. 2001;107(4):477–487. doi: 10.1016/S0092-8674(01)00568-2. [DOI] [PubMed] [Google Scholar]
  4. Edelmann W, Zervas M, Costello P, Roback L, Fischer I, Hammarback JA, Cowan N, Davies P, Wainer B, Kucherlapati R. Neuronal abnormalities in microtubule-associated protein 1B mutant mice. Proc Natl Acad Sci USA. 1996;93:1270–1275. doi: 10.1073/pnas.93.3.1270. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Goddard TD, Kneller DG (2008) SPARKY 3. University of California, San Francisco
  6. Gonzalez-Billault C, Demandt E, Wandosell F, Torres M, Bonaldo P, Stoykova A, Chowdhury K, Gruss P, Avila J, Sánchez MP. Perinatal lethality of microtubule-associated protein 1B-deficient mice expressing alternative isoforms of the protein at low levels. Mol Cell Neurosci. 2000;16(4):408–421. doi: 10.1006/mcne.2000.0880. [DOI] [PubMed] [Google Scholar]
  7. Kazimierczuk K, Zawadzka A, Kozminski W. Narrow peaks and high dimensionalities: exploiting the advantages of random sampling. J Magn Reson. 2009;197(2):219–228. doi: 10.1016/j.jmr.2009.01.003. [DOI] [PubMed] [Google Scholar]
  8. Kazimierczuk K, Zawadzka-Kazimierczuk A, Kozminski W. Non-uniform frequency domain for optimal exploitation of non-uniform sampling. J Magn Reson. 2010;205(2):286–292. doi: 10.1016/j.jmr.2010.05.012. [DOI] [PubMed] [Google Scholar]
  9. Lowery LA, Van Vactor D. The trip of the tip: understanding the growth cone machinery. Nat Rev Mol Cell Biol. 2009;10(5):332–343. doi: 10.1038/nrm2679. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Marley J, Lu M, Bracken C. A method for efficient isotopic labeling of recombinant proteins. J Biomol NMR. 2001;20(1):71–75. doi: 10.1023/A:1011254402785. [DOI] [PubMed] [Google Scholar]
  11. Meixner A, Haverkamp S, Wässle H, Führer S, Thalhammer J, Kropf N, Bittner RE, Lassmann H, Wiche G, Propst F. MAP1B is required for axon guidance and is involved in the development of the central and peripheral nervous system. J Cell Biol. 2000;151:1169–1178. doi: 10.1083/jcb.151.6.1169. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Noiges R, Eichinger R, Kutschera W, Fischer I, Németh Z, Wiche G, Propst F. MAP1A and MAP1B: light chains determine distinct functional properties. J Neurosci. 2002;22(6):2106–2114. doi: 10.1523/JNEUROSCI.22-06-02106.2002. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Noiges R, Stroissnigg H, Trancikova A, Kalny I, Eichinger R, Propst F. Heterotypic complex formation between subunits of microtubule-associated proteins 1A and 1B is due to interaction of conserved domains. Biochim Biophys Acta. 2006;1763(10):1011–1016. doi: 10.1016/j.bbamcr.2006.08.029. [DOI] [PubMed] [Google Scholar]
  14. Opal P, Garcia JJ, Propst F, Matilla A, Orr HT, Zoghbi HY. Mapmodulin/leucine-rich acidic nuclear protein binds the light chain of microtubule-associated protein 1B and modulates neuritogenesis. J Biol Chem. 2003;278(36):34691–34699. doi: 10.1074/jbc.M302785200. [DOI] [PubMed] [Google Scholar]
  15. Orban-Nemeth Z, Simader H, Badurek S, Trancikova A, Propst F. Microtubule-associated protein 1S, a short and ubiquitously expressed member of the microtubule-associated protein 1 family. J Biol Chem. 2005;280(3):2257–2265. doi: 10.1074/jbc.M408984200. [DOI] [PubMed] [Google Scholar]
  16. Schoenfeld TA, McKerracher L, Obar R, Vallee RB. MAP 1A and MAP 1B are structurally related microtubule associated proteins with distinct developmental patterns in the CNS. J Neurosci. 1989;9:1712–1730. doi: 10.1523/JNEUROSCI.09-05-01712.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Stroissnigg H, Trančíková A, Descovich L, Fuhrmann J, Kutschera W, Kostan J, Meixner A, Nothias F, Propst F. S-nitrosylation of microtubule-associated protein 1B mediates nitric oxide induced axon retraction. Nat Cell Biol. 2007;9(9):1035–1045. doi: 10.1038/ncb1625. [DOI] [PubMed] [Google Scholar]
  18. Takei Y, Kondo S, Harada A, Inomata S, Noda T, Hirokawa N. Delayed development of nervous system in mice homozygous for disrupted microtubule-associated protein 1B (MAP1B) gene. J Cell Biol. 1997;137:1615–1626. doi: 10.1083/jcb.137.7.1615. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Tamiola K, Acar B, Mulder FA. Sequence-specific random coil chemical shifts of intrinsically disordered proteins. J Am Chem Soc. 2010;132(51):18000–18003. doi: 10.1021/ja105656t. [DOI] [PubMed] [Google Scholar]
  20. Tögel M, Wiche G, Propst F. Novel features of the light chain of microtubule-associated protein MAP1B: microtubule stabilization, self interaction, actin filament binding, and regulation by the heavy chain. J Cell Biol. 1998;143(3):695–707. doi: 10.1083/jcb.143.3.695. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Tortosa E, Montenegro-Venegas C, Benoist M, Hartel S, Gonzalez-Billault C, Esteban JA, Avila J. Microtubule-associated protein 1B (MAP1B) is required for dendritic spine development and synaptic maturation. J Biol Chem. 2011;286(47):40638–40648. doi: 10.1074/jbc.M111.271320. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Zawadzka-Kazimierczuk A, Kazimierczuk K, Kozminski W. A set of 4D NMR experiments of enhanced resolution for easy resonance assignment in proteins. J Magn Reson. 2010;202(1):109–116. doi: 10.1016/j.jmr.2009.10.006. [DOI] [PubMed] [Google Scholar]
  23. Zawadzka-Kazimierczuk A, Kozminski W, Billeter M. TSAR: a program for automatic resonance assignment using 2D cross-sections of high dimensionality, high-resolution spectra. J Biomol NMR. 2012;54(1):81–95. doi: 10.1007/s10858-012-9652-3. [DOI] [PubMed] [Google Scholar]
  24. Zawadzka-Kazimierczuk A, Kozminski W, Sanderova H, Krasny L. High dimensional and high resolution pulse sequences for backbone resonance assignment of intrinsically disordered proteins. J Biomol NMR. 2012;52(4):329–337. doi: 10.1007/s10858-012-9613-x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Zhang YQ, Bailey AM, Matthies HJ, Renden RB, Smith MA, Speese SD, Rubin GM, Broadie K. Drosophila fragile X-related gene regulates the MAP1B homolog Futsch to control synaptic structure and function. Cell. 2001;107(5):591–603. doi: 10.1016/S0092-8674(01)00589-X. [DOI] [PubMed] [Google Scholar]

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