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. 1994 Dec 15;13(24):5937–5943. doi: 10.1002/j.1460-2075.1994.tb06939.x

A surrogate 15 kDa JC kappa protein is expressed in combination with mu heavy chain by human B cell precursors.

V Francés 1, D Pandrau-Garcia 1, C Guret 1, S Ho 1, Z Wang 1, V Duvert 1, S Saeland 1, H Martinez-Valdez 1
PMCID: PMC395569  PMID: 7813432

Abstract

A novel kappa protein, encoded by a germline JC kappa transcript, is expressed by normal and leukemic human B cell precursors. The transcript displays an open reading frame initiated by a non-AUG codon, and predicts a 15 kDa molecule which could be readily confirmed by in vitro translation. Cellular expression was demonstrated by immunofluorescence, precipitation and Western blotting. Furthermore, 2-D gel electrophoresis revealed that germline JC kappa can covalently associate with mu heavy chain at the surface of pre-B cells. We therefore propose that during B cell lymphopoiesis, two alternative pathways could be operative in which mu heavy chain can either associate with lambda 5 or germ-line JC kappa.

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