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. 2014 Apr 1;141(7):1442–1452. doi: 10.1242/dev.103283

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© 2014. Published by The Company of Biologists Ltd

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Fig. 6. — The Osr1^c/-;Six2^GCE/+ embryos exhibit premature depletion of nephron progenitor cells. (A,B) Whole-mount view of the kidneys from E16.5 Osr1^c/+;Six2^GCE/+ (A) and Osr1^c/-;Six2^GCE/+ (B) littermates. (C-F) Histological analysis of E16.5 kidney sections of Osr1^c/-;Six2^GCE/+ embryos (D,F) and Osr1^c/+;Six2^GCE/+ littermates (C,E). Osr1^c/-;Six2^GCE/+ mutant kidney had lost the nephrogenic zone (F, arrow). (G,H) Immunofluorescence detection of Six2 (green) and pan-cytokeratin (red) in E16.5 Osr1^c/+;Six2^GCE/+ (G) and Osr1^c/-;Six2^GCE/+ (H) kidneys. Scale bars: 100 μm.