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. 2013 Jul 3;154(9):3209–3218. doi: 10.1210/en.2013-1241

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Copyright © 2013 by The Endocrine Society

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/us/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — A reduction in cell viability of AAAS-KD cells. A, There was a significant difference in the total number of cells (by cell counting) on day 5 in the AAAS-KD H295R cells (n = 4) compared with controls after seeding at 2 × 10⁴ cells/well. B, MTS assays on days 2 and 5 with initial seeding density of 5 × 10³ cells/well on day 0. There was a significant reduction in absorbance readings of AAAS-KD H295R cells in comparison with controls on day 2 (n = 3) and day 5 (n = 3). After treatment with 100 μM H₂O₂, a significant reduction in absorbance readings is seen in AAAS-KD H295R cells (n = 3) (C) and AAAS-KD SH-SY5Y cells (n = 4) (D) using MTS assays. There was no reduction in absorbance in untreated AAAS-knockdown SH-SY5Y cells compared with controls. Data represent mean ± SD. *, P < .05; **, P < .01; ***, P < .001.