Figure 3.

Cell cycle arrest, increased apoptosis, and an imbalance of redox homeostasis is observed in AAAS-KD H295R adrenal cells. A, A significant reduction in the percentage cell population in the G₂/mitotic phase of the cell cycle is seen in AAAS-KD H295R cells compared with controls, suggesting cell cycle arrest (n = 3). B, An increase in cleaved relative to total PARP, measured by densitometric analysis after immunoblotting, is observed in H₂O₂-treated AAAS-KD cells compared with controls (n = 3). C, The GSH to GSSG ratio was lower in AAAS-KD cells relative to controls (n = 3). Oxidative stress induced by treatment with 40 μM menadione to control cells further reduces the ratio. RLU, relative light units. Data represent mean ± SD. *, P < .05.