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. 1969 Jan;44(1):27–36. doi: 10.1104/pp.44.1.27

Radioautographic Analysis of the Distribution of Label from 3H-Indoleacetic Acid Supplied to Isolated Coleus Internodes 1

D D Sabnis a,2, G Hirshberg a,3, W P Jacobs a
PMCID: PMC396034  PMID: 16657030

Abstract

Isolated fifth internodes of Coleus blumei Benth. were supplied with 3H-IAA at their apical ends. Microradioautography, using thin sections (0.25-0.5 microns) of Epon embedded tissue and liquid emulsion techniques, revealed extremely specific localization of the acetone-insoluble derivatives of IAA. Changes in the patterns of labeling were followed over periods of 3, 24, and 48 hr at various distances from the source of applied auxin. Progressively basipetal labeling of the cell walls of the youngest xylem elements was evident with time. At the end of 24 hr the label was detected also within the cytoplasm or slime of phloem elements. After 24 to 48 hr, labeling of plastids was evident. The results suggest a continued incorporation of IAA derivatives into cell wall precursors.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Jensen W. A. The Histochemical Localization of Peroxidase in Roots and Its Induction by Indoleacetic Acid. Plant Physiol. 1955 Sep;30(5):426–432. doi: 10.1104/pp.30.5.426. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. LAMOTTE C. E., JACOBS W. P. Quantitative estimation of phloem regeneration in coleus internodes. Stain Technol. 1962 Mar;37:63–73. doi: 10.3109/10520296209114576. [DOI] [PubMed] [Google Scholar]
  3. Leopold A. C., Price C. A. Sulfhydryls in Plants. I. Reactions with Growth Regulators. Plant Physiol. 1957 Nov;32(6):520–525. doi: 10.1104/pp.32.6.520. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Liao S. H., Hamilton R. H. Intracellular localization of growth hormones in plants. Science. 1966 Feb 18;151(3712):822–823. doi: 10.1126/science.151.3712.822. [DOI] [PubMed] [Google Scholar]
  5. SIEGEL S. M., GALSTON A. W. Peroxide genesis in plant tissues and its relation to indoleacetic acid destruction. Arch Biochem Biophys. 1955 Jan;54(1):102–113. doi: 10.1016/0003-9861(55)90012-6. [DOI] [PubMed] [Google Scholar]
  6. Thompson N. P., Jacobs W. P. Polarity of IAA Effect on Sieve-Tube and Xylem Regeneration in Coleus and Tomato Stems. Plant Physiol. 1966 Apr;41(4):673–682. doi: 10.1104/pp.41.4.673. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Torrey J. G. Cellular patterns in developing roots. Symp Soc Exp Biol. 1963;17:285–314. [PubMed] [Google Scholar]
  8. Whitehouse R. L., Zalik S. Autoradiographic evidence of tritiated indolyl-3-acetic acid in epicotyl tissue of Phaseolus coccineus. Experientia. 1967 May 15;23(5):387–388. doi: 10.1007/BF02144534. [DOI] [PubMed] [Google Scholar]

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