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. 1969 Jan;44(1):115–125. doi: 10.1104/pp.44.1.115

The Respiratory Chain of Plant Mitochondria. I. Electron Transport Between Succinate and Oxygen in Skunk Cabbage Mitochondria

Bayard T Storey a, James T Bahr a
PMCID: PMC396048  PMID: 5775846

Abstract

The kinetics of oxidation of ubiquinone, flavoprotein, cytochrome c, and the cytochrome b complex in skunk cabbage (Symplocarpus foetidus) mitochondria made anaerobic with succinate have been measured spectrophotometrically and fluorimetrically in the absence of respiratory inhibitor and in the presence of cyanide or antimycin A. No component identifiable by these means was oxidized rapidly enough in the presence of one or the other inhibitor to qualify for the role of alternate oxidase. Cycles of oxidation and rereduction of flavoprotein and ubiquinone obtained by injecting 12 μm oxygen into the anaerobic mitochondrial suspension were kinetically indistinguishable in the presence of cyanide or antimycin A, implying that these 2 components are part of a respiratory pathway between succinate and oxygen which does not involve the cytochromes and does involve a cyanide-insensitive alternate oxidase. The cytochrome b complex shows biphasic oxidation kinetics with half times of 0.018 sec and 0.4 sec in the absence of inhibitor, which increase to 0.2 sec and 1 sec in the presence of cyanide. In the presence of antimycin A, the oxidation of the cytochrome b complex shows an induction period of 1 sec and a half-time of 3.5 sec. A split respiratory chain with 2 terminal oxidases and a branch point between the cytochromes and flavoprotein and ubiquinone is proposed for these mitochondria.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BENDALL D. S. Cytochromes and some respiratory enzymes in mitochondria from the spadix of Arum maculatum. Biochem J. 1958 Nov;70(3):381–390. doi: 10.1042/bj0700381. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. CHANCE B., REDFEARN E. R. Direct spectrophotometric studies of the kinetics of oxidation and reduction of ubiquinone of heart-muscle particles. Biochem J. 1961 Sep;80:632–644. doi: 10.1042/bj0800632. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CHANCE B. Spectra and reaction kinetics of respiratory pigments of homogenized and intact cells. Nature. 1952 Feb 9;169(4293):215–221. doi: 10.1038/169215a0. [DOI] [PubMed] [Google Scholar]
  4. CHANCE B. The kinetics and inhibition of cytochrome components of the succinic oxidase system. III. Cytochrome b. J Biol Chem. 1958 Nov;233(5):1223–1229. [PubMed] [Google Scholar]
  5. CHANCE B., WILLIAMS G. R. Respiratory enzymes in oxidative phosphorylation. III. The steady state. J Biol Chem. 1955 Nov;217(1):409–427. [PubMed] [Google Scholar]
  6. Chance B., Bonner W. D. Kinetics of Cytochrome Oxidation in Skunk Cabbage Mitochondria. Plant Physiol. 1965 Nov;40(6):1198–1204. doi: 10.1104/pp.40.6.1198. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Chance B., Ernster L., Garland P. B., Lee C. P., Light P. A., Ohnishi T., Ragan C. I., Wong D. Flavoproteins of the mitochondrial respiratory chain. Proc Natl Acad Sci U S A. 1967 May;57(5):1498–1505. doi: 10.1073/pnas.57.5.1498. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Chance B., Hackett D. P. The Electron Transfer System of Skunk Cabbage Mitochondria. Plant Physiol. 1959 Jan;34(1):33–49. doi: 10.1104/pp.34.1.33. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Chance B., Lee C. P., Mela L. Control and conservation of energy in the cytochrome chain. Fed Proc. 1967 Sep;26(5):1341–1354. [PubMed] [Google Scholar]
  10. Chance B. The reactivity of haemoproteins and cytochromes. Biochem J. 1967 Apr;103(1):1–18. doi: 10.1042/bj1030001. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. ESTABROOK R. W. The low temperature spectra of hemoproteins. I. Apparatus and its application to a study of cytochrome c. J Biol Chem. 1956 Dec;223(2):781–794. [PubMed] [Google Scholar]
  12. HIGGINS J. Analysis of sequential reactions. Ann N Y Acad Sci. 1963 May 10;108:305–321. doi: 10.1111/j.1749-6632.1963.tb13382.x. [DOI] [PubMed] [Google Scholar]
  13. Hackett D. P., Haas D. W. Oxidative Phosphorylation and Functional Cytochromes in Skunk Cabbage Mitochondria. Plant Physiol. 1958 Jan;33(1):27–32. doi: 10.1104/pp.33.1.27. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hassinen I., Chance B. Oxidation-reduction properties of the mitochondrial flavoprotein chain. Biochem Biophys Res Commun. 1968 Jun 28;31(6):895–900. doi: 10.1016/0006-291x(68)90536-6. [DOI] [PubMed] [Google Scholar]
  15. Ikuma H., Bonner W. D. Properties of Higher Plant Mitochondria. III. Effects of Respiratory Inhibitors. Plant Physiol. 1967 Nov;42(11):1535–1544. doi: 10.1104/pp.42.11.1535. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. KING T. E. RECONSTITUTION OF RESPIRATORY CHAIN ENZYME SYSTEMS. XI. USE OF ARTIFICIAL ELECTRON ACCEPTORS IN THE ASSAY OF SUCCINATE-DEHYDROGENATING ENZYMES. J Biol Chem. 1963 Dec;238:4032–4036. [PubMed] [Google Scholar]
  17. Lance C., Bonner W. D. The respiratory chain components of higher plant mitochondria. Plant Physiol. 1968 May;43(5):756–766. doi: 10.1104/pp.43.5.756. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Storey B. T., Bahr J. T. The respiratory chain of plant mitochondria. II. Oxidative phosphorylation in skunk cabbage mitochondria. Plant Physiol. 1969 Jan;44(1):126–134. doi: 10.1104/pp.44.1.126. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Storey B. T. Determination of ubiquinone in electron transport particles from beef heart. Arch Biochem Biophys. 1966 Jun;114(3):431–437. doi: 10.1016/0003-9861(66)90364-x. [DOI] [PubMed] [Google Scholar]
  20. Storey B. T. Rate of ubiquinone oxidation in electron transport particles reduced by succinate. Arch Biochem Biophys. 1968 Aug;126(2):585–592. doi: 10.1016/0003-9861(68)90445-1. [DOI] [PubMed] [Google Scholar]
  21. YONETANI T., RAY G. S. STUDIES ON CYTOCHROME OXIDASE. VI. KINETICS OF THE AEROBIC OXIDATION OF FERROCYTOCHROME C BY CYTOCHROME OXIDASE. J Biol Chem. 1965 Aug;240:3392–3398. [PubMed] [Google Scholar]
  22. Yocum C. S., Hackett D. P. Participation of Cytochromes in the Respiration of the Aroid Spadix. Plant Physiol. 1957 May;32(3):186–191. doi: 10.1104/pp.32.3.186. [DOI] [PMC free article] [PubMed] [Google Scholar]

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