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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: Yeast. 2013 Dec 13;31(3):103–112. doi: 10.1002/yea.2992

Figure 5.

Figure 5

MATALPHA1 mutants are α-specific sterile but can mate as MATa cells with low efficiency. Plates were incubated at 30C. (A) Halo assay to measure α-factor production. Cells were patched onto a lawn of MATa bar1 cells on a YPD plate and incubated for one day. (B) Mating as MATα cells. Each of the five strains were mixed 1:1 with a MATa lys1 tester strain in YPD broth and spotted onto a YPD plate. The master plate was photographed after 1 day and then the cells were replica plated to selective medium, which was photographed after 1 day. (C) Mating as MATa cells. Similar to (B), except the five strains were mixed with a MATα lys1 tester strain. Mating was photographed after 1 and 5 days incubation.