Table 1.

PCR Primers

Name	Purpose	Sequence (5′ to 3′)
U2	Forward primer to amplify URA3 from pJH136	CGTACGCTGCAGGTCGAC
D2	Reverse primer to amplify URA3 from pJH136	ATCGATGAATTCGAGCTCG
al1.46.1	50/50 primer for α1∆	TATGAAATGTATCAACCATATATAATAACTTAATAGAC GACATTCACAATAGTGTGGTCGTGGCGGAGGTTGTTTAT CTTTCGAGTACTGAATGTTGTCACGTACGCTGCAGGTCG ACa
al1.46.2	50/50 primer for α1-2x	TATCAACCATATATAATAACTTAATAGACGACATTCAC AATATGTTTACTCGAGCCTGCTTTCAAAATTAAGAACAA AGCATCCAAATCATACAGAAACACACGTACGCTGCAGG TCGACb
al1.46.3	Reverse primer for α1∆ and α1-2x	TGTGTTTCTGTATGATTTGGATGCTTTGTTCTTAATTTTG AAAGCAGGCTATCGATGAATTCGAGCTCGc
al1.52.1	Forward primer for α1::kanMX4	CTTCACTTTTTATGAAATGTATCAACCATATATAATAAC TTAATAGACGACATTCACAATCGTACGCTGCAGGTCGA Cd
al1.52.2	Reverse primer for α1::kanMX4	GCGGAAAGCTGAAACTAAAAGAAAAACCCGACTATGCT ATTTTAATCATTGAAAACGAATATCGATGAATTCGAGCT CGe
URA3.54.1	Amplify URA3 to make pJH136	CGTACGCTGCAGGTCGACTGTGGTTTCAGGGTCCATAA AGf
URA3.54.3	Amplify URA3 to make pJH136	ATCGATGAATTCGAGCTCGGGTAATAACTGATATAATT AAATTGAAGCg
URA3.for	Forward primer for split-URA3 PCR	CACAGTTAAGCCGCTAAAGGC
URA3.rev	Reverse primer for split-URA3 PCR	AGTATATTCTCCAGTAGCTAGGGAGCC

^aMATALPHA1 −50 to −1, double underline; 50 nts following stop codon, dotted underline; U2, single underline.

^bMATALPHA1 −41 to +9, double underline; 2 nt deletion and XhoI site, italics; +14 to +63, dotted underline; U2, single underline.

^cReverse complement of MATALPHA1 +14 to +63, dotted underline; D2, single underline.

^dMATALPHA1 −60 to −1, double underline; U2, single underline.

^eReverse complement of MATALPHA1 60 nts following stop codon, double underline; D2, single underline.

^fU2, single underline.

^gD2, single underline.