Abstract
Disc electrophoresis was used to separate water soluble proteins from hardy, non-hardy, and frost killed cabbage (Brassica oleracea var. capitata) leaves. Amidoschwarz staining failed to reveal any new bands as a result of hardening although the relative amounts of proteins in individual bands changed. Sulfhydryl groups in the protein bands were stained with 2,2-dihydroxy-6,6-dinaphthyl disulfide and labeled with 14C p-chloromercuribenzoate. Significant decreases in the sulfhydryl content of the total water soluble protein were found during hardening and as a result of frost death. The decrease during hardening was paralleled by a significant increase in the water soluble protein. There was a significant increase in the sulfhydryl content per unit high molecular weight protein but a decrease in the sulfhydryl content per total protein as a result of frost death. This was interpreted as evidence for intermolecular disulfide bond formation during freezing.
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Selected References
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- Heber U. W., Santarius K. A. Loss of Adenosine Triphosphate Synthesis Caused by Freezing and Its Relationship to Frost Hardiness Problems. Plant Physiol. 1964 Sep;39(5):712–719. doi: 10.1104/pp.39.5.712. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kohn H., Levitt J. Frost Hardiness Studies on Cabbage Grown under Controlled Conditions. Plant Physiol. 1965 May;40(3):476–480. doi: 10.1104/pp.40.3.476. [DOI] [PMC free article] [PubMed] [Google Scholar]
- STEWARD F. C., LYNDON R. F., BARBER J. T. ACRYLAMIDE GEL ELECTROPHORESIS OF SOLUBLE PLANT PROTEINS: A STUDY ON PEA SEEDLINGS IN RELATION TO DEVELOPMENT. Am J Bot. 1965 Feb;52:155–164. [PubMed] [Google Scholar]