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. 1969 Mar;44(3):351–360. doi: 10.1104/pp.44.3.351

Photoreaction of Manganese Catalyst in Photosynthetic Oxygen Evolution

G M Cheniae a, I F Martin a
PMCID: PMC396091  PMID: 16657069

Abstract

The formation of active O2 evolving centers following addition of Mn2+ to Mn deficient Anacystis nidulans cells yielded an estimate of 6 to 12 Mn atoms associated with each O2 evolving reaction center. Restoration of activity upon addition of Mn ions is affected in 3 ways: (1) Stimulation of the uptake of exogenous Mn into the cells—this uptake occurs in darkness, but is enhanced 5 to 10 fold by light; a high concentration of DCMU (1 × 10−5m) decreases this light enhanced influx no more than 50 to 75%; (2) Photoreactivation of the O2 evolving centers, after excess Mn has been accumulated in the cells essentially no increase in Hill activity is observed unless the cells are illuminated. This photoreactivation is fully inhibited by 10−6m DCMU and partially by benzoquinone. The Q10 of photoreactivation proper is close to 1; (3) Photoinhibition of the activation—photoreactivation occurs most effectively in weak intensities (< one-fiftieth photosynthetic saturation in normal cells). Apparently at higher intensities an inhibitory photoprocess is overriding. This inhibition proved reversible. The photoreactivation leads to new stable O2 evolving centers as evidenced by an increase in the rate at saturating intensity, quantum yield, and the O2 gush.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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