Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1969 Apr;44(4):508–516. doi: 10.1104/pp.44.4.508

Fat Metabolism in Higher Plants. XXXVII. Characterization of the β-Oxidation Systems From Maturing and Germinating Castor Bean Seeds 1

D Hutton a, P K Stumpf a
PMCID: PMC396118  PMID: 16657093

Abstract

In the maturing castor bean seed (Ricinus communis), maximum β-oxidation appears at 28 days after flowering and in the germinating seed, 4 days after germination. Highest specific activities for both β-oxidation systems and their component enzymes are associated with cytosomal particles banding at a density of 1.25 g/ml in a sucrose gradient. Substrate specificity studies indicate that of several fatty acids, ricinoleate is oxidized most rapidly by the preparation from the maturing seed (28 days after flowering) while palmitate and linoleate are oxidized most rapidly by extracts obtained from tissue germinated for 4 days. The β-oxidation activities observed in both systems reflect the expression of activity of at least 3 of the component enzymes, crotonase, β-hydroxyacyl dehydrogenase and β-keto-thiolase, which rise and fall co-ordinately. Acyl thiokinase does not appear to play a limiting role in regulating β-oxidation per se under the conditions employed here.

Full text

PDF
508

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BEERS R. F., Jr, SIZER I. W. A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. J Biol Chem. 1952 Mar;195(1):133–140. [PubMed] [Google Scholar]
  2. BEEVERS H. Metabolic production of sucrose from fat. Nature. 1961 Jul 29;191:433–436. doi: 10.1038/191433a0. [DOI] [PubMed] [Google Scholar]
  3. BERNHARD W., ROUILLER C. Microbodies and the problem of mitochondrial regeneration in liver cells. J Biophys Biochem Cytol. 1956 Jul 25;2(4 Suppl):355–360. doi: 10.1083/jcb.2.4.355. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Breidenbach R. W., Beevers H. Association of the glyoxylate cycle enzymes in a novel subcellular particle from castor bean endosperm. Biochem Biophys Res Commun. 1967 May 25;27(4):462–469. doi: 10.1016/s0006-291x(67)80007-x. [DOI] [PubMed] [Google Scholar]
  5. Breidenbach R. W., Kahn A., Beevers H. Characterization of glyoxysomes from castor bean endosperm. Plant Physiol. 1968 May;43(5):705–713. doi: 10.1104/pp.43.5.705. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Brooks J. L., Stumpf P. K. Fat metabolism in higher plants. XXXIX. Properties of a soluble fatty acid synthesizing system from lettuce chloroplasts. Arch Biochem Biophys. 1966 Sep 26;116(1):108–116. doi: 10.1016/0003-9861(66)90019-1. [DOI] [PubMed] [Google Scholar]
  7. De Duve C., Baudhuin P. Peroxisomes (microbodies and related particles). Physiol Rev. 1966 Apr;46(2):323–357. doi: 10.1152/physrev.1966.46.2.323. [DOI] [PubMed] [Google Scholar]
  8. Galliard T., Stumpf P. K. Fat metabolism in higher plants. 30. Enzymatic synthesis of ricinoleic acid by a microsomal preparation from developing Ricinus communis seeds. J Biol Chem. 1966 Dec 25;241(24):5806–5812. [PubMed] [Google Scholar]
  9. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  10. Overath P., Raufuss E. M. The induction of the enzymes of fatty acid degradation in Escherichia coli. Biochem Biophys Res Commun. 1967 Oct 11;29(1):28–33. doi: 10.1016/0006-291x(67)90535-9. [DOI] [PubMed] [Google Scholar]
  11. RACKER E. Spectrophotometric measurements of the enzymatic formation of fumaric and cis-aconitic acids. Biochim Biophys Acta. 1950 Jan;4(1-3):211–214. doi: 10.1016/0006-3002(50)90026-6. [DOI] [PubMed] [Google Scholar]
  12. Yamada M., Stumpf P. K. Fat metabolism in higher plants. XXIV. A soluble beta-oxidative system from germinating seeds of Ricinus communis. Plant Physiol. 1965 Jul;40(4):653–658. doi: 10.1104/pp.40.4.653. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. ZELITCH I., OCHOA S. Oxidation and reduction of glycolic and glyoxylic acids in plants. I. Glycolic and oxidase. J Biol Chem. 1953 Apr;201(2):707–718. [PubMed] [Google Scholar]
  14. ZELITCH I. Oxidation and reduction of glycolic and glyoxylic acids in plants. II. Glyoxylic acid reductase. J Biol Chem. 1953 Apr;201(2):719–726. [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES