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. 1969 Apr;44(4):527–534. doi: 10.1104/pp.44.4.527

Energy and Electron Transfer Systems of Chlamydomonas reinhardi. I. Photosynthetic and Respiratory Cytochrome Systems of the Pale Green Mutant 1

Tetsuo Hiyama a, Mitsuo Nishimura a, Britton Chance a
PMCID: PMC396121  PMID: 16657096

Abstract

The role of cytochromes in photosynthetic electron transfer system has been studied using the pale green mutant of Chlamydomonas reinhardi (ATCC 18302). The existence of cytochromes b563 and f is confirmed, while no significant amount of ascorbate-reducible cytochrome b559 is detected in this mutant. The presence of cytochrome c and a small amount of a-type cytochrome is determined in these cells.

Light-induced oxidation of cytochrome b563 is eliminated by oxygen-induced oxidation, and oxygen-induced oxidation is greatly diminished under illumination. Antimycin A diminishes the oxygen-induced oxidation of cytochrome b563, but does not affect light-induced oxidation. In the aerobic state in the presence of 2-heptyl-4-hydroxyquinoline-N-oxide, cytochrome b563 is reduced by illumination with far red light; this reduction is not inhibited by 3-(4′-chlorophenyl)-1,1-dimethylurea.

A tentative scheme for the electron transfer system in chloroplasts, which involves a cyclic pathway via cytochrome b563 and its interaction with oxygen, is proposed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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