Abstract
Action spectra were obtained for photosystems I and II in chemically fixed algal cells and for photosystem I in unfixed lysozyme treated cells. Untreated algal cells yielded neither of the 2 light reactions with the reaction mixtures used. The action spectra for photosystem I in the blue-green alga Anacystis nidulans and red alga Porphyridium cruentum follow the absorption spectrum of chlorophyll a with a small peak in the region of the accessory pigments. In the green alga Chlorella pyrenoidosa the photosystem I action spectrum follows the absorption spectrum of chlorophyll a. Photosystem II action spectra in A. nidulans and P. cruentum follow the absorption spectra of the accessory pigments while that in C. pyrenoidosa is shifted slightly toward the blue spectral region. These results provide additional evidence that formaldehyde fixed cells are valid models for studying the light reactions of photosynthesis.
Full text
PDF



Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Biggins J. Photosynthetic Reactions by Lysed Protoplasts and Particle Preparations from the Blue-Green Alga, Phormidium luridum. Plant Physiol. 1967 Oct;42(10):1447–1456. doi: 10.1104/pp.42.10.1447. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Biggins J. Preparation of Metabolically Active Protoplasts and Particle Preparations from the Blue-Green Alga, Phormidium luridum. Plant Physiol. 1967 Oct;42(10):1442–1446. doi: 10.1104/pp.42.10.1442. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DUYSENS L. N., AMESZ J., KAMP B. M. Two photochemical systems in photosynthesis. Nature. 1961 May 6;190:510–511. doi: 10.1038/190510a0. [DOI] [PubMed] [Google Scholar]
- HAXO F. T., BLINKS L. R. Photosynthetic action spectra of marine algae. J Gen Physiol. 1950 Mar;33(4):389–422. doi: 10.1085/jgp.33.4.389. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hallier U. W., Park R. B. Photosynthetic Light Reactions in Chemically Fixed Anacystis nidulans, Chlorella pyrenoidosa, and Porphyridium cruentum. Plant Physiol. 1969 Apr;44(4):535–539. doi: 10.1104/pp.44.4.535. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Joliot P., Joliot A., Kok B. Analysis of the interactions between the two photosystems in isolated chloroplasts. Biochim Biophys Acta. 1968 Apr 2;153(3):635–652. doi: 10.1016/0005-2728(68)90191-6. [DOI] [PubMed] [Google Scholar]
- MYERS J., FRENCH C. S. Evidences from action spectra for a specific participation of chlorophyll b in photosynthesis. J Gen Physiol. 1960 Mar;43:723–736. doi: 10.1085/jgp.43.4.723. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nishimura M. Energy-and electron-transfer systems in algal photosynthesis. II. Oxidation-reduction reactions of two cytochromes and interactions of two photochemical systems in red algae. Biochim Biophys Acta. 1968 May 28;153(4):838–847. doi: 10.1016/0005-2728(68)90010-8. [DOI] [PubMed] [Google Scholar]
- SUSOR W. A., KROGMANN D. W. HILL ACTIVITY IN CELL-FREE PREPARATIONS OF A BLUE-GREEN ALGA. Biochim Biophys Acta. 1964 Jul 29;88:11–19. doi: 10.1016/0926-6577(64)90150-0. [DOI] [PubMed] [Google Scholar]
- Sauer K., Biggins J. Action spectra and quantum yields for nicotinamide--adenine dinucleotide phosphate reduction by chloroplasts. Biochim Biophys Acta. 1965 May 25;102(1):55–72. doi: 10.1016/0926-6585(65)90202-5. [DOI] [PubMed] [Google Scholar]
- Sauer K., Park R. B. The Hill reaction of chloroplasts. Action spectra and quantum requirements. Biochemistry. 1965 Dec;4(12):2791–2798. doi: 10.1021/bi00888a032. [DOI] [PubMed] [Google Scholar]