Abstract
An alkali-insoluble glucan synthesized from UDP-d-glucose by the particulate enzyme system from Phaseolus aureus is hydrolyzed by a highly purified exo-β-(1 → 3)-d-glucanase to d-glucose, to the extent of 91% in 24 hr.
The alkali-insoluble glucan from GDP-d-glucose formed by the particulate enzyme system from the same plant which is known to be (from chemical data) a β-(1 → 4)-d-glucan (cellulose) is not acted upon by this glucanase.
Full text
PDF



Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BANDURSKI R. S., AXELROD B. The chromatographic identification of some biologically important phosphate esters. J Biol Chem. 1951 Nov;193(1):405–410. [PubMed] [Google Scholar]
- BARBER H. A., ELBEIN A. D., HASSID W. Z. THE SYNTHESIS OF CELLULOSE BY ENZYME SYSTEMS FROM HIGHER PLANTS. J Biol Chem. 1964 Dec;239:4056–4061. [PubMed] [Google Scholar]
- Brummond D. O., Gibbons A. P. Enzymatic cellulose synthesis from UDP-(14C)-glucose by Lupinus albus. Biochem Z. 1965 Aug 6;342(3):308–318. [PubMed] [Google Scholar]
- FEINGOLD D. S., NEUFELD E. F., HASSID W. Z. Synthesis of a beta-1, 3-linked glucan by extracts of Phaseolus aureus seedlings. J Biol Chem. 1958 Oct;233(4):783–788. [PubMed] [Google Scholar]
- Flowers H. M., Batra K. K., Kemp J., Hassid W. Z. Biosynthesis of Insoluble Glucans From Uridine-Diphosphate-d-Glucose With Enzyme Preparations From Phaseolus aureus and Lupinus albus. Plant Physiol. 1968 Oct;43(10):1703–1709. doi: 10.1104/pp.43.10.1703. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HANES C. S., ISHERWOOD F. A. Separation of the phosphoric esters on the filter paper chromatogram. Nature. 1949 Dec 31;164(4183):1107-12, illust. doi: 10.1038/1641107a0. [DOI] [PubMed] [Google Scholar]
- Huotari F. I., Nelson T. E., Smith F., Kirkwood S. Purification of an exo-beta-D-(1 bonded to 3)-glucanase from Basidiomycete species QM 806. J Biol Chem. 1968 Mar 10;243(5):952–956. [PubMed] [Google Scholar]
- Leloir L. F. Nucleoside diphosphate sugars and saccharide synthesis. Biochem J. 1964 Apr;91(1):1–8. [PubMed] [Google Scholar]
- Ordin L., Hall M. A. Cellulose synthesis in higher plants from UDP glucose. Plant Physiol. 1968 Mar;43(3):473–476. doi: 10.1104/pp.43.3.473. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ordin L., Hall M. A. Studies on Cellulose Synthesis by a Cell-free Oat Coleoptile Enzyme System: Inactivation by Airborne Oxidants. Plant Physiol. 1967 Feb;42(2):205–212. doi: 10.1104/pp.42.2.205. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Villemez C. L., Franz G., Hassid W. Z. Biosynthesis of Alkali Insoluble Polysaccharide from UDP-d-Glucose with Particulate Enzyme Preparations from Phaseolus aureus. Plant Physiol. 1967 Sep;42(9):1219–1223. doi: 10.1104/pp.42.9.1219. [DOI] [PMC free article] [PubMed] [Google Scholar]
