Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1969 Jul;44(7):946-949, 951-954. doi: 10.1104/pp.44.7.946

Photosynthesis, Photorespiration and Respiration of Chloroplasts From Acetabularia mediterrania1

R G S Bidwell a,2, W B Levin a, D C Shephard a
PMCID: PMC396195  PMID: 16657162

Abstract

A chloroplast fraction isolated from Acetabularia mediterrania carries on photosynthesis at rates essentially equal to those of whole cells. Electron and phase contrast microscopy reveals that the chloroplasts are intact and well preserved. Preparations contain no identifiable peroxisomes, but some cytoplasmic and mitochondrial contamination is present. Photosynthesis and CO2 production in light by chloroplast preparations are in many respects similar to that of bean leaves, although the measured rates are somewhat lower. Respiration and photosynthesis of chloroplast preparations and whole cells of Acetabularia is essentially similar except that cells have a strong dark-type respiration which continues in light and is CO2 dependent, the substrate being mainly recent photosynthate. The data suggest that chloroplasts are the site of photorespiration.

Full text

PDF
946

Images in this article

949Image
on p.949

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arnon D. I. COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS. Plant Physiol. 1949 Jan;24(1):1–15. doi: 10.1104/pp.24.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BIDWELL R. G. Direct paper chromatography of soluble compounds in small samples of tissue adhering to the paper. Can J Biochem Physiol. 1962 Jun;40:757–761. [PubMed] [Google Scholar]
  3. Forrester M. L., Krotkov G., Nelson C. D. Effect of oxygen on photosynthesis, photorespiration and respiration in detached leaves. I. Soybean. Plant Physiol. 1966 Mar;41(3):422–427. doi: 10.1104/pp.41.3.422. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Frederick S. E., Newcomb E. H. Microbody-like organelles in leaf cells. Science. 1969 Mar 21;163(3873):1353–1355. doi: 10.1126/science.163.3873.1353. [DOI] [PubMed] [Google Scholar]
  5. Hew C. S., Krotkov G. Effect of Oxygen on the Rates of CO(2) Evolution in Light and in Darkness by Photosynthesizing and Non-Photosynthesizing Leaves. Plant Physiol. 1968 Mar;43(3):464–466. doi: 10.1104/pp.43.3.464. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kisaki T., Tolbert N. E. Glycolate and glyoxylate metabolism by isolated peroxisomes or chloroplasts. Plant Physiol. 1969 Feb;44(2):242–250. doi: 10.1104/pp.44.2.242. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Shephard D. C., Levin W. B., Bidwell R. G. Normal photosynthesis by isolated chloroplasts. Biochem Biophys Res Commun. 1968 Aug 13;32(3):413–420. doi: 10.1016/0006-291x(68)90677-3. [DOI] [PubMed] [Google Scholar]
  8. Tolbert N. E., Oeser A., Kisaki T., Hageman R. H., Yamazaki R. K. Peroxisomes from spinach leaves containing enzymes related to glycolate metabolism. J Biol Chem. 1968 Oct 10;243(19):5179–5184. [PubMed] [Google Scholar]
  9. Tolbert N. E., Oeser A., Yamazaki R. K., Hageman R. H., Kisaki T. A survey of plants for leaf peroxisomes. Plant Physiol. 1969 Jan;44(1):135–147. doi: 10.1104/pp.44.1.135. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Walker D. A., Baldry C. W., Cockburn W. Photosynthesis by isolated chloroplasts, simultaneous measurement of carbon assimilation and oxygen evolution. Plant Physiol. 1968 Sep;43(9):1419–1422. doi: 10.1104/pp.43.9.1419. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES