Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1969 Jul;44(7):990-993, 995, 997-1000. doi: 10.1104/pp.44.7.990

Chloroplast Ultrastructure in Mutant Strains of Chlamydomonas reinhardi Lacking Components of the Photosynthetic Apparatus 1

Ursula W Goodenough a, R P Levine a
PMCID: PMC396203  PMID: 16657170

Abstract

The fine structure of the chloroplast of wild-type and 9 photosynthetic mutant strains of Chlamydomonas reinhardi is described. The chloroplast phenotypes of the mutant strains are clearly distinct from the wild type in all but 2 cases. Moreover, strains with similar photosynthetic disabilities have structurally similar chloroplasts. These differences are apparently not the result of altered chlorophyll content, nor of photosynthetic inactivity. It is therefore proposed that the structural alterations are in some way related to the mutant strains' inability to synthesize active components of the photosynthetic electron transport chain.

Full text

PDF
990

Images in this article

993Image
on p.993
995Image
on p.995

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arnon D. I. COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS. Plant Physiol. 1949 Jan;24(1):1–15. doi: 10.1104/pp.24.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bachmann M. D., Robertson D. S., Bowen C. C., Anderson I. C. Chloroplast development in pigment deficient mutants of maize. I. Structural anomalies in plastids of allelic mutants at the w3 locus. J Ultrastruct Res. 1967 Nov;21(1):41–60. doi: 10.1016/s0022-5320(67)80005-4. [DOI] [PubMed] [Google Scholar]
  3. Chua N. H., Levine R. P. The photosynthetic electron transport chain of Chlamydomonas reinhardi. 8. The 520 nm light-induced absorbance change in the wild-type and mutant strains. Plant Physiol. 1969 Jan;44(1):1–6. doi: 10.1104/pp.44.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. GIBBS S. P. Chloroplast development in Ochromonas danica. J Cell Biol. 1962 Nov;15:343–361. doi: 10.1083/jcb.15.2.343. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. GIBBS S. P. The ultrastructure of the chloroplasts of algae. J Ultrastruct Res. 1962 Dec;7:418–435. doi: 10.1016/s0022-5320(62)90038-2. [DOI] [PubMed] [Google Scholar]
  6. Givan A. L., Levine R. P. The photosynthetic electron transport chain of Chlamydomonas reinhardi. VII. Photosynthetic phosphorylation by a mutant strain of Chlamydomonas reinhardi deficient in active P700. Plant Physiol. 1967 Sep;42(9):1264–1268. doi: 10.1104/pp.42.9.1264. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gorman D. S., Levine R. P. Cytochrome f and plastocyanin: their sequence in the photosynthetic electron transport chain of Chlamydomonas reinhardi. Proc Natl Acad Sci U S A. 1965 Dec;54(6):1665–1669. doi: 10.1073/pnas.54.6.1665. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gorman D. S., Levine R. P. Photosynthetic Electron Transport Chain of Chlamydomonas reinhardi VI. Electron Transport in Mutant Strains Lacking Either Cytochrome 553 or Plastocyanin. Plant Physiol. 1966 Dec;41(10):1648–1656. doi: 10.1104/pp.41.10.1648. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gorman D. S., Levine R. P. Photosynthetic Electron Transport Chain of Chlamydomonas reinhardi. IV. Purification and Properties of Plastocyanin. Plant Physiol. 1966 Dec;41(10):1637–1642. doi: 10.1104/pp.41.10.1637. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gorman D. S., Levine R. P. Photosynthetic Electron Transport Chain of Chlamydomonas reinhardi. V. Purification and Properties of Cytochrome 553 and Ferredoxin. Plant Physiol. 1966 Dec;41(10):1643–1647. doi: 10.1104/pp.41.10.1643. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Johnson U. G., Porter K. R. Fine structure of cell division in Chlamydomonas reinhardi. Basal bodies and microtubules. J Cell Biol. 1968 Aug;38(2):403–425. doi: 10.1083/jcb.38.2.403. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. LEVINE R. P., SMILLIE R. M. THE PHOTOSYNTHETIC ELECTRON TRANSPORT CHAIN OF CHLAMYDOMONAS REINHARDI. I. TRIPHOSPHOPYRIDINE NUCLEOTIDE PHOTOREDUCTION IN WILD-TYPE AND MUTANT STRAINS. J Biol Chem. 1963 Dec;238:4052–4057. [PubMed] [Google Scholar]
  13. Lavorel J. Fluorescence Properties of Wild-Type Chlamydomonas reinhardi and Three Mutant Strains Having Impaired Photosynthesis. Plant Physiol. 1968 Jul;43(7):1049–1055. doi: 10.1104/pp.43.7.1049. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Levine R. P. Genetic dissection of photosynthesis. Science. 1968 Nov 15;162(3855):768–771. doi: 10.1126/science.162.3855.768. [DOI] [PubMed] [Google Scholar]
  15. Levine R. P., Gorman D. S. Photosynthetic electron transport chain of Chlamydomonas reinhardi. 3. Light-induced absorbance changes in chloroplast fragments of the wild type and mutant strains. Plant Physiol. 1966 Oct;41(8):1293–1300. doi: 10.1104/pp.41.8.1293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Ohad I., Siekevitz P., Palade G. E. Biogenesis of chloroplast membranes. I. Plastid dedifferentiation in a dark-grown algal mutant (Chlamydomonas reinhardi). J Cell Biol. 1967 Dec;35(3):521–552. doi: 10.1083/jcb.35.3.521. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Ringo D. L. Flagellar motion and fine structure of the flagellar apparatus in Chlamydomonas. J Cell Biol. 1967 Jun;33(3):543–571. doi: 10.1083/jcb.33.3.543. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. SMILLIE R. M., LEVINE R. P. THE PHOTOSYNTHETIC ELECTRON TRANSPORT CHAIN OF CHLAMYDOMONAS REINHARDI. II. COMPONENTS OF THE TRIPHOSPHOPYRIDINE NUCLEOTIDE-REDUCTIVE PATHWAY IN WILD-TYPE AND MUTANT STRAINS. J Biol Chem. 1963 Dec;238:4058–4062. [PubMed] [Google Scholar]
  19. YOTSUYANAGI Y. [Study of yeast mitochondria. II. Mitochondria of respiration-deficient mutants]. J Ultrastruct Res. 1962 Aug;7:141–158. doi: 10.1016/s0022-5320(62)80032-x. [DOI] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES