Abstract
Effect of salinity on malate dehydrogenase activity was studied.
Pea root tips contain 2 different malate dehydrogenases. One is located in the particulate, mitochondrial fraction, the other in the soluble, cytoplasmic fraction. Both can act when coupled with either NAD or NADP.
Growing plants in Na2SO4 salinated medium did not affect the pattern of the malate dehydrogenases in the root tips. Growing plants in NaCl salinated media resulted in the appearance of a new, third isoenzyme. This new isoenzyme was located in the cytoplasmic fraction.
Salinity of both types, when present in growth medium, induced increases in the NADP coupled activity of the mitochondrial malate dehydrogenase. The NAD coupled activity, however, was depressed except in the cytoplasmic fraction of plants grown in media salinated with NaCl to 1 atmosphere. Addition of either of the salts to assay media of enzymes, isolated from plants grown in non salinated substrate, did not have any significant effect.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- DAVIES D. D. The specificity of malic dehydrogenase from higher plants. Biochem J. 1961 Jul;80:93–99. doi: 10.1042/bj0800093. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hiatt A. J., Evans H. J. Influence of Salts on Activity of Malic Dehydrogenase from Spinach Leaves. Plant Physiol. 1960 Sep;35(5):662–672. doi: 10.1104/pp.35.5.662. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hiatt A. J. Relationship of Cell Sap pH to Organic Acid Change During Ion Uptake. Plant Physiol. 1967 Feb;42(2):294–298. doi: 10.1104/pp.42.2.294. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KAPLAN N. O., CIOTTI M. M. Evolution and differentiation of dehvdrogenases. Ann N Y Acad Sci. 1961 Nov 2;94:701–722. doi: 10.1111/j.1749-6632.1961.tb35567.x. [DOI] [PubMed] [Google Scholar]
- Kahane I., Poljakoff-Mayber A. Effect of substrate salinity on the ability for protein synthesis in pea roots. Plant Physiol. 1968 Jul;43(7):1115–1119. doi: 10.1104/pp.43.7.1115. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Lips S. H., Beevers H. Compartmentation of Organic Acids in Corn Roots II. The Cytoplasmic Pool of Malic Acid. Plant Physiol. 1966 Apr;41(4):713–717. doi: 10.1104/pp.41.4.713. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lips S. H., Beevers H. Compartmentation of organic acids in corn roots I. Differential labeling of 2 malate pools. Plant Physiol. 1966 Apr;41(4):709–712. doi: 10.1104/pp.41.4.709. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Price C. A., Thimann K. V. Dehydrogenase Activity and Respiration; a Quantitative Comparison. Plant Physiol. 1954 Nov;29(6):495–500. doi: 10.1104/pp.29.6.495. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ting I. P., Sherman I. W., Dugger W. M. Intracellular location and possible function of malic dehydrogenase isozymes from young maize root tissue. Plant Physiol. 1966 Jun;41(6):1083–1084. doi: 10.1104/pp.41.6.1083. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Weimberg R. An electrophoretic analysis of the isozymes of malate dehydrogenase in several different plants. Plant Physiol. 1968 Apr;43(4):622–628. doi: 10.1104/pp.43.4.622. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Weimberg R. Effect of sodium chloride on the activity of a soluble malate dehydrogenase from pea seeds. J Biol Chem. 1967 Jun 25;242(12):3000–3006. [PubMed] [Google Scholar]