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. 1969 Aug;44(8):1187–1193. doi: 10.1104/pp.44.8.1187

Synthesis and Secretion of Hydroxyproline Containing Macromolecules in Carrots. I. Kinetic Analysis

Maarten J Chrispeels a,1
PMCID: PMC396237  PMID: 16657187

Abstract

When disks of carrot (Daucus carota) phloem parenchyma are incubated for 6 days there is a 10-fold increase in cell wall hydroxyproline due to the synthesis and secretion of hydroxyproline-containing macromolecules. The synthesis of these molecules and their secretion are demonstrated by measuring the kinetics of incorporation and of chase of 14C-proline and hydroxyproline in different fractions of the cytoplasm and the cell wall. The hydroxyproline-containing molecules which are secreted are associated with the membranous organelles of the cytoplasm. They can be fractionated into trichloroacetic acid-soluble and trichloroacetic acid-precipitated fractions. The properties of the trichloroacetic acid-soluble fraction associated with the membranous organelles are consistent with its role as a cell wall precursor.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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