Figure 4. Global DNA methylation. Global methylation of hepatic DNA was similar in fetal livers of C3H and tx-j mice at baseline without maternal choline treatment. Tx-j mouse fetal livers from maternal choline treated mice had increased global DNA methylation compared with livers from untreated mice, whereas among choline supplemented groups at 21d, tx-j livers had increased DNA methylation compared with livers of C3H mice (*P < 0.01). Each dot for each genotype/treatment group represents an individual sample from one representative mouse.