Figure 3. (A) Scatterplots of qMSP analysis for candidate gene promoters in the Validation cohort (normal n = 19, cancer n = 30). The relative level of methylated DNA for each gene in each sample was determined as a ratio of MSP for the amplified gene to β-actin. Red line denotes cut-off value. (B) Scatterplots of qMSP analysis of FKBP6, INTS1, and ZNF516 in the Prevalence cohort (normal n = 18, cancer n = 90). The relative level of methylated DNA for each gene in each sample was determined as a ratio of MSP for the amplified gene to β-actin. Red line denotes cut-off value.