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. 1996 Apr 16;93(8):3450–3454. doi: 10.1073/pnas.93.8.3450

Development of a bovine X chromosome linkage group and painting probes to assess cattle, sheep, and goat X chromosome segment homologies.

F A Ponce de Leon 1, S Ambady 1, G A Hawkins 1, S M Kappes 1, M D Bishop 1, J M Robl 1, C W Beattie 1
PMCID: PMC39629  PMID: 8622956

Abstract

The X chromosome linkage group is conserved in placental mammals. However, X chromosome morphological differences, due to internal chromosome rearrangements, exist among mammalian species. We have developed bovine chromosome painting probes for Xp and Xq to assess segment homologies between the submetacentric bovine X chromosome and the acrocentric sheep and goat X chromosomes. These painting probes and their corresponding DNA libraries were developed by chromosome micromanipulation, DNA micropurification, microcloning, and PCR amplification. The bovine Xp painting probe identified an interstitially located homologous segment in the sheep and goat Xq region, most probably resulting from chromosome inversion. Ten type II (microsatellite) markers obtained from the bovine Xq library and five other X chromosome assigned, but unlinked, markers were used to generate a linkage map for Xq spanning 89.4 centimorgans. The chromosome painting probes and molecular markers generated in this study would be useful for comparative mapping and tracing of internal X chromosome rearrangements in all ruminant species and would contribute to the understanding of mammalian sex chromosome evolution.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bishop M. D., Kappes S. M., Keele J. W., Stone R. T., Sunden S. L., Hawkins G. A., Toldo S. S., Fries R., Grosz M. D., Yoo J. A genetic linkage map for cattle. Genetics. 1994 Feb;136(2):619–639. doi: 10.1093/genetics/136.2.619. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bumstead N., Palyga J. A preliminary linkage map of the chicken genome. Genomics. 1992 Jul;13(3):690–697. doi: 10.1016/0888-7543(92)90143-g. [DOI] [PubMed] [Google Scholar]
  3. Crawford A. M., Dodds K. G., Ede A. J., Pierson C. A., Montgomery G. W., Garmonsway H. G., Beattie A. E., Davies K., Maddox J. F., Kappes S. W. An autosomal genetic linkage map of the sheep genome. Genetics. 1995 Jun;140(2):703–724. doi: 10.1093/genetics/140.2.703. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Di Berardino D., Iannuzzi L. Detailed description of R-banded bovine chromosomes. J Hered. 1982 Nov-Dec;73(6):434–438. doi: 10.1093/oxfordjournals.jhered.a109693. [DOI] [PubMed] [Google Scholar]
  5. Eggen A., Fries R. An integrated cytogenetic and meiotic map of the bovine genome. Anim Genet. 1995 Aug;26(4):215–236. doi: 10.1111/j.1365-2052.1995.tb03249.x. [DOI] [PubMed] [Google Scholar]
  6. Ellegren H., Chowdhary B. P., Johansson M., Marklund L., Fredholm M., Gustavsson I., Andersson L. A primary linkage map of the porcine genome reveals a low rate of genetic recombination. Genetics. 1994 Aug;137(4):1089–1100. doi: 10.1093/genetics/137.4.1089. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Goudarzi K., Ciampolini R., Vaiman D., Leveziel H. A new bovine dinucleotide repeat microsatellite: microsatellite INRA 18. Anim Genet. 1993 Jun;24(3):221–221. [PubMed] [Google Scholar]
  8. Hayes H., Petit E., Dutrillaux B. Comparison of RBG-banded karyotypes of cattle, sheep, and goats. Cytogenet Cell Genet. 1991;57(1):51–55. doi: 10.1159/000133114. [DOI] [PubMed] [Google Scholar]
  9. Kaftanovskaya H. M., Serov O. L. High-resolution GTG-banded chromosomes of cattle, sheep, and goat: a comparative study. J Hered. 1994 Sep-Oct;85(5):395–400. doi: 10.1093/oxfordjournals.jhered.a111485. [DOI] [PubMed] [Google Scholar]
  10. Kappes S. M., Bishop M. D., Keele J. W., Penedo M. C., Hines H. C., Grosz M. D., Hawkins G. A., Stone R. T., Sunden S. L., Beattie C. W. Linkage of bovine erythrocyte antigen loci B, C, L, S, Z, R' and T' and the serum protein loci post-transferrin 2 (PTF 2), vitamin D binding protein (GC) and albumin (ALB) to DNA microsatellite markers. Anim Genet. 1994 Jun;25(3):133–140. doi: 10.1111/j.1365-2052.1994.tb00101.x. [DOI] [PubMed] [Google Scholar]
  11. Keele J. W., Wray J. E., Behrens D. W., Rohrer G. A., Sunden S. L., Kappes S. M., Bishop M. D., Stone R. T., Alexander L. J., Beattie C. W. A conceptual database model for genomic research. J Comput Biol. 1994 Spring;1(1):65–76. doi: 10.1089/cmb.1994.1.65. [DOI] [PubMed] [Google Scholar]
  12. Lander E. S., Green P. Construction of multilocus genetic linkage maps in humans. Proc Natl Acad Sci U S A. 1987 Apr;84(8):2363–2367. doi: 10.1073/pnas.84.8.2363. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Levin I., Santangelo L., Cheng H., Crittenden L. B., Dodgson J. B. An autosomal genetic linkage map of the chicken. J Hered. 1994 Mar-Apr;85(2):79–85. doi: 10.1093/oxfordjournals.jhered.a111427. [DOI] [PubMed] [Google Scholar]
  14. Lichter P., Cremer T., Borden J., Manuelidis L., Ward D. C. Delineation of individual human chromosomes in metaphase and interphase cells by in situ suppression hybridization using recombinant DNA libraries. Hum Genet. 1988 Nov;80(3):224–234. doi: 10.1007/BF01790090. [DOI] [PubMed] [Google Scholar]
  15. Lichter P., Tang C. J., Call K., Hermanson G., Evans G. A., Housman D., Ward D. C. High-resolution mapping of human chromosome 11 by in situ hybridization with cosmid clones. Science. 1990 Jan 5;247(4938):64–69. doi: 10.1126/science.2294592. [DOI] [PubMed] [Google Scholar]
  16. Lüdecke H. J., Senger G., Claussen U., Horsthemke B. Cloning defined regions of the human genome by microdissection of banded chromosomes and enzymatic amplification. Nature. 1989 Mar 23;338(6213):348–350. doi: 10.1038/338348a0. [DOI] [PubMed] [Google Scholar]
  17. O'Brien S. J. Mammalian genome mapping: lessons and prospects. Curr Opin Genet Dev. 1991 Jun;1(1):105–111. doi: 10.1016/0959-437x(91)80050-v. [DOI] [PubMed] [Google Scholar]
  18. O'Brien S. J., Womack J. E., Lyons L. A., Moore K. J., Jenkins N. A., Copeland N. G. Anchored reference loci for comparative genome mapping in mammals. Nat Genet. 1993 Feb;3(2):103–112. doi: 10.1038/ng0293-103. [DOI] [PubMed] [Google Scholar]
  19. Rohrer G. A., Alexander L. J., Keele J. W., Smith T. P., Beattie C. W. A microsatellite linkage map of the porcine genome. Genetics. 1994 Jan;136(1):231–245. doi: 10.1093/genetics/136.1.231. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Saunders R. D., Glover D. M., Ashburner M., Siden-Kiamos I., Louis C., Monastirioti M., Savakis C., Kafatos F. PCR amplification of DNA microdissected from a single polytene chromosome band: a comparison with conventional microcloning. Nucleic Acids Res. 1989 Nov 25;17(22):9027–9037. doi: 10.1093/nar/17.22.9027. [DOI] [PMC free article] [PubMed] [Google Scholar]

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