Skip to main content
Plant Physiology logoLink to Plant Physiology
. 1969 Nov;44(11):1523–1527. doi: 10.1104/pp.44.11.1523

Polygalactolipids in Spinach Chloroplast 1

D E Webster a, S B Chang a,2
PMCID: PMC396299  PMID: 5397496

Abstract

Two polygalactolipids, designated as components A and B, were isolated from spinach chloroplasts and were also obtained from glycolipid products synthesized with chloroplast enzymes using uridine diphosphate galactose as a galactose donor. These lipids were purified by column and thin layer chromatography. Chemical analysis of component A indicates that the lipid is trigalactosyl diglyceride, whereas component B behaves like tetragalactosyl diglyceride on a thin layer plate. The major fatty acid in trigalactosyl diglyceride was α-linolenic acid. Relative amount (molar ratio) of galactolipids in spinach chloroplasts was monogalactosyl diglyceride:digalactosyl diglyceride:trigalactosyl diglyceride:(tetragalactosyl diglyceride) = 60:30:5:1.

Full text

PDF
1523

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arnon D. I. COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS. Plant Physiol. 1949 Jan;24(1):1–15. doi: 10.1104/pp.24.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. HANAHAN D. J., OLLEY J. N. Chemical nature of monophosphoinositides. J Biol Chem. 1958 Apr;231(2):813–828. [PubMed] [Google Scholar]
  3. JACIN H., MISHKIN A. R. SEPARATION OF CARBOHYDRATES ON BORATE-IMPREGNATED SILICA GEL G PLATES. J Chromatogr. 1965 Apr;18:170–173. doi: 10.1016/s0021-9673(01)80341-1. [DOI] [PubMed] [Google Scholar]
  4. LEVIN E., LENNARZ W. J., BLOCH K. OCCURRENCE AND LOCALIZATION OF ALPHA-LINOLENIC ACID CONTAINING GALACTOLIPIDS IN THE PHOTOSYNTHETIC APPARATUS OF ANABAENA VARIABILLIS. Biochim Biophys Acta. 1964 Aug 5;84:471–474. doi: 10.1016/0926-6542(64)90015-0. [DOI] [PubMed] [Google Scholar]
  5. Lennarz W. J., Talamo B. The chemical characterization and enzymatic synthesis of mannolipids in Micrococcus lysodeikticus. J Biol Chem. 1966 Jun 10;241(11):2707–2719. [PubMed] [Google Scholar]
  6. MARUO B., BENSON A. A. Cyclic glycerophosphate formation from the glycerolphosphatides. J Biol Chem. 1959 Feb;234(2):254–256. [PubMed] [Google Scholar]
  7. Neufeld E. F., Hall C. W. Formation of galactolipids by chloroplasts. Biochem Biophys Res Commun. 1964;14:503–508. doi: 10.1016/0006-291x(64)90259-1. [DOI] [PubMed] [Google Scholar]
  8. Ongun A., Mudd J. B. Biosynthesis of galactolipids in plants. J Biol Chem. 1968 Apr 10;243(7):1558–1566. [PubMed] [Google Scholar]
  9. PARK R. B., PON N. G. Chemical composition and the substructure of lamellae isolated from Spinacea oleracea chloroplasts. J Mol Biol. 1963 Feb;6:105–114. doi: 10.1016/s0022-2836(63)80126-6. [DOI] [PubMed] [Google Scholar]
  10. Prottey C., Ballou C. E. Diacyl myoinositol monomannoside from Propionibacterium shermanii. J Biol Chem. 1968 Dec 10;243(23):6196–6201. [PubMed] [Google Scholar]
  11. RAPPORT M. M., ALONZO N. Photometric determination of fatty acid ester groups in phospholipides. J Biol Chem. 1955 Nov;217(1):193–198. [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES