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. 1970 Jan;45(1):37–40. doi: 10.1104/pp.45.1.37

The Effects of Abscisic Acid on Growth and Nucleic Acid Synthesis in Excised Embryonic Bean Axes 1

D C Walton a, G S Soofi a,2, E Sondheimer a
PMCID: PMC396350  PMID: 16657275

Abstract

Abscisic acid (ABA) is an effective inhibitor of cell elongation in excised embryonic bean axes whether added prior to or after the initiation of cell elongation. Zeatin partially reverses this growth inhibition. ABA inhibits 32P incorporation into ribosomal RNA, transfer RNA, and DNA but not into the tenaciously bound fraction of elongating axes in a manner resembling 5-fluorouracil, a compound which does not inhibit axis growth. The methylated albumin on kie-selguhr elution profiles of nucleic acids obtained from axes treated with either ABA, 5-fluorouracil, or a combination of the two are similar, and zeatin treatment has little apparent effect on these results. Our results suggest that the inhibition of growth in the axes by ABA is not due to its inhibition of DNA synthesis.

ABA (1.9 × 10−5m) inhibits growth by 30 and 70% within 1 and 2 hours, respectively, after its addition to elongating axes. Its kinetics of inhibition are similar to those obtained with cycloheximide, and both compounds are more effective than 8-azaadenine. Based on these results, it is suggested that one possible effect of ABA may be at the level of translation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CHERRY J. H. ASSOCIATION OF RAPIDLY METABOLIZED DNA AND RNA. Science. 1964 Nov 20;146(3647):1066–1069. doi: 10.1126/science.146.3647.1066. [DOI] [PubMed] [Google Scholar]
  2. Cherry J. H., Chroboczek H., Carpenter W. J., Richmond A. Nucleic Acid Metabolism in Peanut Cotyledons. Plant Physiol. 1965 May;40(3):582–587. doi: 10.1104/pp.40.3.582. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chrispeels M. J., Varner J. E. Hormonal control of enzyme synthesis: on the mode of action of gibberellic Acid and abscisin in aleurone layers of barley. Plant Physiol. 1967 Jul;42(7):1008–1016. doi: 10.1104/pp.42.7.1008. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. De Koning A. J. Estimation of sphingomyelin. Nature. 1965 May 15;206(985):715–716. doi: 10.1038/206715b0. [DOI] [PubMed] [Google Scholar]
  5. Haber A. H., Foard D. E., Perdue S. W. Actions of gibberellic and abscisic acids on lettuce seed germination without actions on nuclear DNA synthesis. Plant Physiol. 1969 Mar;44(3):463–467. doi: 10.1104/pp.44.3.463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Pearson J. A., Wareing P. F. Effect of abscisic acid on activity of chromatin. Nature. 1969 Feb 15;221(5181):672–673. doi: 10.1038/221672a0. [DOI] [PubMed] [Google Scholar]
  7. RALPH R. K., BELLAMY A. R. ISOLATION AND PURIFICATION OF UNDEGRADED RIBONUCLEIC ACIDS. Biochim Biophys Acta. 1964 May 18;87:9–16. doi: 10.1016/0926-6550(64)90041-6. [DOI] [PubMed] [Google Scholar]
  8. SUEOKA N., CHENG T. Y. Fractionation of nucleic acids with the methylated albumin column. J Mol Biol. 1962 Mar;4:161–172. doi: 10.1016/s0022-2836(62)80048-5. [DOI] [PubMed] [Google Scholar]
  9. Tepper H. B., Hollis C. A., Galson E. C., Sondheimer E. Germination of Excised Fraxinus ornus Embryos With and Without Phleomycin. Plant Physiol. 1967 Nov;42(11):1493–1496. doi: 10.1104/pp.42.11.1493. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Van Overbeek J., Loeffler J. E., Mason M. I. Dormin (Abscisin II), inhibitor of plant DNA synthesis? Science. 1967 Jun 16;156(3781):1497–1499. doi: 10.1126/science.156.3781.1497. [DOI] [PubMed] [Google Scholar]
  11. Walton D. C. Germination of phaseolus vulgaris I. Resumption of axis growth. Plant Physiol. 1966 Feb;41(2):298–302. doi: 10.1104/pp.41.2.298. [DOI] [PMC free article] [PubMed] [Google Scholar]

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