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. 1970 Jan;45(1):41–45. doi: 10.1104/pp.45.1.41

The Influence of Auxin and Ethylene on Chromatin-directed Ribonucleic Acid Synthesis in Soybean Hypocotyl 1,2

Robert E Holm a,3, T J O'Brien a, J L Key a,4, J H Cherry a
PMCID: PMC396351  PMID: 16657277

Abstract

Soybean seedlings treated with ethylene exhibited small increases in ribonucleic acid content in the elongating section of the hypocotyl. Chromatin isolated from the elongating section of ethylene-treated seedlings showed a 35 to 60% increase in the capacity for RNA synthesis. The ethylene-induced response was saturated at 1 microliter/liter of ethylene and was fully expressed after 3 hours. Auxin caused marked accumulation of RNA and DNA in the elongating and basal tissue of the hypocotyl. Chromatin isolated from these auxin-treated tissues showed an 8- to 10- fold increase in RNA synthetic capacity as measured in vitro. Ethylene added with auxin reduced the auxin enhancement of nucleic acid synthesis in the elongating and basal tissues. Both ethylene and auxin treatment of the seedlings inhibited nucleic acid accumulation and chromatin activity in the apical tissue. Ethylene did not appear to mediate the auxin effects on nucleic acid synthesis in soybean hypocotyl with the possible exception of inhibition in the apical tissue.

The RNA which was synthesized by chromatin isolated from control and auxin- and ethylene-treated tissues was characterized by nearest neighbor analyses. The nearest neighbor frequencies of the RNA products synthesized by chromatin isolated from auxin- and ethylene-treated hypocotyl tissue were different from each other and different from the control RNA product.

Seedlings treated in sealed containers exhibited growth, RNA, and DNA responses, especially to ethylene, different from those of seedlings treated in continuous flow containers.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956 Feb;62(2):315–323. doi: 10.1042/bj0620315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Burg S. P., Burg E. A. The interaction between auxin and ethylene and its role in plant growth. Proc Natl Acad Sci U S A. 1966 Feb;55(2):262–269. doi: 10.1073/pnas.55.2.262. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. HUANG R. C., BONNER J. Histone, a suppressor of chromosomal RNA synthesis. Proc Natl Acad Sci U S A. 1962 Jul 15;48:1216–1222. doi: 10.1073/pnas.48.7.1216. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Key J. L., Shannon J. C. Enhancement by Auxin of Ribonucleic Acid Synthesis in Excised Soybean Hypocotyl Tissue. Plant Physiol. 1964 May;39(3):360–364. doi: 10.1104/pp.39.3.360. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. O'Brien T. J., Jarvis B. C., Cherry J. H., Hanson J. B. Enhancement by 2,4-dichlorophenoxyacetic acid of chromatin RNA polymerase in soybean hypocotyl tissue. Biochim Biophys Acta. 1968 Nov 20;169(1):35–43. doi: 10.1016/0005-2787(68)90006-3. [DOI] [PubMed] [Google Scholar]
  6. Travers A. A., Burgessrr Cyclic re-use of the RNA polymerase sigma factor. Nature. 1969 May 10;222(5193):537–540. doi: 10.1038/222537a0. [DOI] [PubMed] [Google Scholar]

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