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. 1970 Feb;45(2):148–152. doi: 10.1104/pp.45.2.148

Deuterium Oxide as a Density Label of Peroxidases in Germinating Barley Embryos 1

Wayne Anstine 1,2, J V Jacobsen 1,2,2, J G Scandalios 1,2,3, J E Varner 1,2,3
PMCID: PMC396371  PMID: 16657293

Abstract

Density labeling with deuterium oxide, gel electrophoresis, and isopycnic equilibrium sedimentation were used to study the appearance and disappearance of individual peroxidases in the embryos of germinating barley. No detectable label was incorporated into those peroxidases which are present in the embryo of the dry seed and disappear during germination. Deuterium was incorporated into the additional peroxidases which appeared in the embryo during germination. This incorporation is not due to deuterium-hydrogen exchange into preformed proteins. The results indicate that the newly appearing peroxidases arise by synthesis during germination.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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