Abstract
A suspension culture of soybean (Glycine max L.) was grown on a defined medium in which the nitrogen sources were nitrate (25 mM) and ammonium (2 mM). The cells did not grow on nitrate unless the medium was supplemented with ammonium or glutamine. The l- and d-isomers of 12 amino acids tested singly could not replace ammonium. Most amino acids (4 mM) inhibited growth when the cells were cultured on nitrate and ammonium. Cells from five other plants (Reseda luteoli L.; Triticum monococcum L.; flax, Linum usitatissimum L.; horseradish, Amoracia lapathifolia Gilib; Haplopappus gracilis L.) grew on the defined medium with nitrate (25 mM) as the sole nitrogen source. Higher cell yields were obtained when ammonium (2 mM) or glutamine also was present. Supplementing the defined medium with high concentrations of ammonium (20 mM) inhibited growth of soybean, Haplopappus, and wheat cells. Addition of citrate (5 mM) relieved the inhibitory effects of ammonium in soybean and wheat cells but not in the Haplopappus cells.
Full text
PDFSelected References
These references are in PubMed. This may not be the complete list of references from this article.
- Crofts A. R. Amine uncoupling of energy transfer in chloroplasts. I. Relation to ammonium ion uptake. J Biol Chem. 1967 Jul 25;242(14):3352–3359. [PubMed] [Google Scholar]
- Earle E. D., Torrey J. G. Morphogenesis in cell colonies grown from Convolvulus cell suspensions plated on synthetic media. Am J Bot. 1965 Oct;52(9):891–899. [PubMed] [Google Scholar]
- Filner P. Regulation of nitrate reductase in cultured tobacco cells. Biochim Biophys Acta. 1966 May 5;118(2):299–310. doi: 10.1016/s0926-6593(66)80038-3. [DOI] [PubMed] [Google Scholar]
- Filner P. Semi-conservative replication of DNA in a higher plant cell. Exp Cell Res. 1965 Aug;39(1):33–39. doi: 10.1016/0014-4827(65)90004-2. [DOI] [PubMed] [Google Scholar]
- Gamborg O. L. Aromatic metabolism in plants. II. Enzymes of the shikimate pathway in suspension cultures of plant cells. Can J Biochem. 1966 Jun;44(6):791–799. doi: 10.1139/o66-097. [DOI] [PubMed] [Google Scholar]
- Gamborg O. L., Eveleigh D. E. Culture methods and detection of glucanases in suspension cultures of wheat and barley. Can J Biochem. 1968 May;46(5):417–421. doi: 10.1139/o68-063. [DOI] [PubMed] [Google Scholar]
- Gamborg O. L., Miller R. A., Ojima K. Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res. 1968 Apr;50(1):151–158. doi: 10.1016/0014-4827(68)90403-5. [DOI] [PubMed] [Google Scholar]
- Gibson F., Pittard J., Reich E. Ammonium ions as the source of nitrogen for tryptophan biosynthesis in whole cells of Escherichia coli. Biochim Biophys Acta. 1967 Apr 25;136(3):573–576. doi: 10.1016/0304-4165(67)90020-7. [DOI] [PubMed] [Google Scholar]
- Holzer H., Mecke D., Wulff K., Liess K., Heilmeyer L., Jr Metabolite-induced enzymatic inactivation of glutamine synthetase in E. coli. Adv Enzyme Regul. 1967;5:211–225. doi: 10.1016/0065-2571(67)90017-9. [DOI] [PubMed] [Google Scholar]
- Long C. W., Pardee A. B. Cytidine triphosphate synthetase of Escherichia coli B. I. Purification and kinetics. J Biol Chem. 1967 Oct 25;242(20):4715–4721. [PubMed] [Google Scholar]
- Reinert J., Tazawa M., Semenoff S. Nitrogen compounds as factors of embryogenesis in vitro. Nature. 1967 Dec 23;216(5121):1215–1216. doi: 10.1038/2161215a0. [DOI] [PubMed] [Google Scholar]