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. 1970 Apr;45(4):395–400. doi: 10.1104/pp.45.4.395

Temporal and Hormonal Control of β-1,3-Glucanase in Phaseolus vulgaris L

F B Abeles 1, L E Forrence 1
PMCID: PMC396421  PMID: 5427109

Abstract

The endo-β-1, 3-glucanase (β-1, 3-glucan 3-glucanhydrolase, EC 3.2.1.6) extracted from Phaseolus vulgaris L. cv. Red Kidney had a pH optimum of 5 and a temperature optimum of 50 C. Excision of plant tissue resulted in an increase in β-1, 3-glucanase activity after a 6-hour lag period. The increase could be prevented by indole-3-acetic acid, gibberellic acid, and cytokinins. Ethylene (half-maximal concentration = 0.1 microliter/liter) promoted the synthesis of β-1, 3-glucanase, and 10% CO2 overcame some of the ethylene effect. Cycloheximide prevented the induction of β-1, 3-glucanase, but actinomycin D and chromomycin A3 had only a partial effect.

The amount of callose in sieve tube cells correlated with levels of β-1, 3-glucanase, suggesting that this enzyme played a role in the degradation of β-1, 3-glucans.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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