Abstract
An enzyme, which lacks cellobiase activity, responsible for the synthesis of ethyl-β-glucoside has been found in the extracts of pea hooks (1-centimeter length of the apical portion of epicotyl) and has been partially purified by ammonium sulfate fractionation. The enzyme can transfer the glucosyl moiety from a group of phenolic β-glucosides to ethanol. A specific β-glucosyl donor, isosuccinimide β-glucoside, isolated from the extracts of pea seedlings shows the highest activity. The characteristics of the enzyme which synthesizes ethyl-β-glucoside and the glucosyl donor, isosuccinimide β-glucoside, have been studied. The significance of this system (enzyme and isosuccinimide β-glucoside) has been discussed.
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- HASH J. H., KING K. W. On the nature of the beta-glucosidases of Myrothecium verrucaria. J Biol Chem. 1958 May;232(1):381–393. [PubMed] [Google Scholar]
- HASH J. H., KING K. W. Some properties of an aryl-beta-glucosidase from culture filtrates of Myrothecium verrucaria. J Biol Chem. 1958 May;232(1):395–402. [PubMed] [Google Scholar]
- Kocourek J., Bucharová V., Buchbauerová V., Jirácek V., Kostír J. A., Kostír J. V., Kysilka C., Mostková I., Pribylová A., Tichá M. Glycosides V. Pisatoside: the new alkali-labile, nitrogenous beta-D-glucopyranoside of pea (pisum sativum L). Arch Biochem Biophys. 1967 Aug;121(2):531–532. doi: 10.1016/0003-9861(67)90112-9. [DOI] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Liu T. Y., Castelfrance P. A. Isosuccinimide-beta-glucoside, the glucosyl donor in the synthesis of ethyl-beta-glucoside by pea seedling extracts. Arch Biochem Biophys. 1968 Mar 11;123(3):645–646. doi: 10.1016/0003-9861(68)90188-4. [DOI] [PubMed] [Google Scholar]
- Liu T. Y., Oppenheim A., Castelfranco P. Ethyl alcohol metabolism in leguminous seedlings. Plant Physiol. 1965 Nov;40(6):1261–1268. doi: 10.1104/pp.40.6.1261. [DOI] [PMC free article] [PubMed] [Google Scholar]
- VITEK V. SUGAR-LIKE, DIPHENYLAMINE-POSITIVE METABOLITES IN PEA SEEDLINGS. Biochim Biophys Acta. 1964 Nov 8;93:429–432. doi: 10.1016/0304-4165(64)90400-3. [DOI] [PubMed] [Google Scholar]