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. 2014 Apr;20(4):568–579. doi: 10.1261/rna.043513.113

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© 2014 Zhang et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 3.0 Unported), as described at http://creativecommons.org/licenses/by-nc/3.0/.

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FIGURE 5. — Far-Western dot-blot assay studying the role of each subregion of AnaRneC in AnaPnp–AnaRneC interaction. AnaRneC and its subregion deletion variants were dotted onto duplicate nitrocellulose membranes. Subsequently, one of the membranes was incubated with 2 µM AnaPnp and the other one was not. Both membranes were finally subjected to immunodetection with the antibody against AnaPnp (see Materials and Methods for details). The deleted regions in AnaRneC variants are illustrated with boxes with gray stripes. BSA and AnaPnp were used as the negative control and the positive control, respectively.