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. 1970 Jun;45(6):679–683. doi: 10.1104/pp.45.6.679

Some Spectral Properties of Pea Phytochrome in Vivo and in Vitro

Marylee S Everett a,1, Winslow R Briggs a,2
PMCID: PMC396492  PMID: 16657373

Abstract

The transformation difference spectrum for phytochrome (Pr spectrum minus Pfr spectrum) in pea tissue is determined below 560 nanometers and compared with similar data on phytochrome in vitro The difference spectrum in vivo between phytochrome intermediates and Pfr is also shown for comparison with the data on phytochrome solutions. These comparisons show that the peaks in the spectra occurring in the blue wave lengths are shifted to shorter wave lengths and are much enhanced when phytochrome is extracted from the cell and placed in solution. The results indicate that the physicochemical state of phytochrome in the cell may be different from that of the extracted pigment.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Briggs W. R. Long-lived Intermediates in Phytochrome Transformation I: In Vitro Studies. Plant Physiol. 1969 Aug;44(8):1081–1088. doi: 10.1104/pp.44.8.1081. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Briggs W. R. Long-lived Intermediates in Phytochrome Transformation II: In Vitro and In Vivo Studies. Plant Physiol. 1969 Aug;44(8):1089–1094. doi: 10.1104/pp.44.8.1089. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Briggs W. R., Siegelman H. W. Distribution of Phytochrome in Etiolated Seedlings. Plant Physiol. 1965 Sep;40(5):934–941. doi: 10.1104/pp.40.5.934. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Briggs W. R., Zollinger W. D., Platz B. B. Some Properties of Phytochrome Isolated From Dark-grown Oat Seedlings (Avena sativa L.). Plant Physiol. 1968 Aug;43(8):1239–1243. doi: 10.1104/pp.43.8.1239. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. KLEIN S. The effect of low temperature on the development of the lamellar system in chloroplasts. J Biophys Biochem Cytol. 1960 Oct;8:529–538. doi: 10.1083/jcb.8.2.529. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Mumford F. E., Jenner E. L. Purification and characterization of phytochrome from oat seedlings. Biochemistry. 1966 Nov;5(11):3657–3662. doi: 10.1021/bi00875a039. [DOI] [PubMed] [Google Scholar]
  7. Pratt L. H., Briggs W. R. Photochemical and Nonphotochemical Reactions of Phytochrome in vivo. Plant Physiol. 1966 Mar;41(3):467–474. doi: 10.1104/pp.41.3.467. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. SHIBATA K., BENSON A. A., CALVIN M. The absorption spectra of suspensions of living micro-organisms. Biochim Biophys Acta. 1954 Dec;15(4):461–470. doi: 10.1016/0006-3002(54)90002-5. [DOI] [PubMed] [Google Scholar]
  9. SIEGELMAN H. W., FIRER E. M. PURIFICATION OF PHYTOCHROME FROM OAT SEEDLINGS. Biochemistry. 1964 Mar;3:418–423. doi: 10.1021/bi00891a019. [DOI] [PubMed] [Google Scholar]
  10. Yentsch C. S., Guillard R. R. The absorption of chlorophyll-beta in vivo. Photochem Photobiol. 1969 Apr;9(4):385–388. doi: 10.1111/j.1751-1097.1969.tb07303.x. [DOI] [PubMed] [Google Scholar]

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