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. 1970 Jun;45(6):719–722. doi: 10.1104/pp.45.6.719

Biosynthesis of Cytidine Diphosphate Diglyceride by Enzyme Preparations from Cauliflower 1

S Sumida a, J B Mudd a
PMCID: PMC396500  PMID: 16657380

Abstract

The activity of the enzyme responsible for the synthesis of cytidine diphosphate diglyceride has been found in the 20,000g pellet of a cauliflower (Brassica oleracea) inflorescence homogenate. Greater enzyme activity was found in the pellet after treatment with 0.02% Triton X-100, or after freezing and thawing. Activity was greater when fluoride was included in the reaction mixture. The enzyme has a pH optimum of 5.6, an optimal Mn2+ concentration of 1mm and an optimal Mg2+ concentration of 30 mm. At the optimal concentration, Mn2+ was 4 times better than Mg2+. The utilization of biosynthesized cytidine diphosphate diglyceride can be enhanced by addition of inositol, thereby causing the formation of phosphatidyl inositol.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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