Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1970 Jul;46(1):45–49. doi: 10.1104/pp.46.1.45

Chemical Constitution of the Host-Specific Toxin of Helminthosporium carbonum1

Ross B Pringle a
PMCID: PMC396531  PMID: 5481091

Abstract

The host-specific toxin of Helminthosporium carbonum Ullstrup has a molecular formula approximating C32H50N6O10. The compound has been crystallized and a crystalline hydrochloride derivative has been produced. The molecular weight, as determined by chromatography on Sephadex G-10, is slightly less than 700. The toxin appears to be a cyclic peptide, since, although it does not react with ninhydrin or dinitrofluorobenzene, it yields, on hydrolysis, compounds which react to these reagents. It is unstable in dilute acids, yielding ninhydrin-reacting products. Complete acid hydrolysis yields alanine, proline, and three other ninhydrin-reacting components. The infrared spectrum of the toxin reveals an ester band in addition to amide absorption. Its ultraviolet spectrum reveals the presence of unsaturation in the molecule. The toxin is relatively unstable and loses its specific toxicity. This loss of activity appears to be associated with loss of nitrogen and with decreased solubility in water.

Full text

PDF
45

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bodanszky M., Perlman D. Peptide antibiotics. Science. 1969 Jan 24;163(3865):352–358. doi: 10.1126/science.163.3865.352. [DOI] [PubMed] [Google Scholar]
  2. Bycroft B. W. Structural relationships in microbial peptides. Nature. 1969 Nov 8;224(5219):595–597. doi: 10.1038/224595a0. [DOI] [PubMed] [Google Scholar]
  3. Gross E., Morell J. L., Craig L. C. Dehydroalanyllysine: identical COOH-terminal structures in the peptide antibiotics nisin and subtilin. Proc Natl Acad Sci U S A. 1969 Mar;62(3):952–956. doi: 10.1073/pnas.62.3.952. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Meehan F., Murphy H. C. A New Helminthosporium Blight of Oats. Science. 1946 Nov 1;104(2705):413–414. doi: 10.1126/science.104.2705.413. [DOI] [PubMed] [Google Scholar]
  5. NELSON L. S. KAMPAC. J Kans Med Soc. 1964 Dec;65:602–603. [PubMed] [Google Scholar]
  6. PRINGLE R. B., BRAUN A. C. Constitution of the toxin of Helminthosporium victoriae. Nature. 1958 Apr 26;181(4617):1205–1206. doi: 10.1038/1811205a0. [DOI] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES