Abstract
When abscission in leaf explants from Phaseolus vulgaris, cultivar Red Kidney, was allowed to proceed while the explants were in 2H2O, a 1.25% increase in the buoyant density of cellulase in a cesium chloride gradient was observed. These data indicate that the increase in cellulase activity during abscission is a result of the synthesis of new protein. Two differentially soluble forms of cellulase are present in the abscission zone. The form which is soluble only in a high salt buffer seems more closely related to the abscission process than the form which is soluble in dilute buffer. The correlation between changes in pull force and increase in cellulase activity and the effects of several hormones on cellulase activity are discussed.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Abeles F. B. Abscission: role of cellulase. Plant Physiol. 1969 Mar;44(3):447–452. doi: 10.1104/pp.44.3.447. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Almin K. E., Eriksson K. E., Jansson C. Enzymic degradation of polymers. II. Viscometric determination of cellulase activity in absolute terms. Biochim Biophys Acta. 1967 Jul 11;139(2):248–253. doi: 10.1016/0005-2744(67)90029-0. [DOI] [PubMed] [Google Scholar]
- Datko A. H., Maclachlan G. A. Indoleacetic Acid and the synthesis of glucanases and pectic enzymes. Plant Physiol. 1968 May;43(5):735–742. doi: 10.1104/pp.43.5.735. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fan D. F., Maclachlan G. A. Massive synthesis of ribonucleic Acid and cellulase in the pea epicotyl in response to indoleacetic Acid, with and without concurrent cell division. Plant Physiol. 1967 Aug;42(8):1114–1122. doi: 10.1104/pp.42.8.1114. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Filner P., Varner J. E. A test for de novo synthesis of enzymes: density labeling with H2O18 of barley alpha-amylase induced by gibberellic acid. Proc Natl Acad Sci U S A. 1967 Oct;58(4):1520–1526. doi: 10.1073/pnas.58.4.1520. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Longo C. P. Evidence for de novo synthesis of isocitratase and malate synthesis in germinating peanut cotyledons. Plant Physiol. 1968 Apr;43(4):660–664. doi: 10.1104/pp.43.4.660. [DOI] [PMC free article] [PubMed] [Google Scholar]