Skip to main content
Plant Physiology logoLink to Plant Physiology
. 1970 Sep;46(3):363–366. doi: 10.1104/pp.46.3.363

Synthesis and Secretion of Hypoxyproline-containing Macromolecules in Carrots

III. Metabolic Requirements for Secretion 1

Marcia R Doerschug a, Maarten J Chrispeels a
PMCID: PMC396597  PMID: 16657468

Abstract

Using pulse-chase experiments with radioactive proline, it is possible to study the rapid transfer from the cytoplasm to the cell wall of the hydroxyproline-rich protein found in the cell walls of higher plants. The secretion of this protein is not obligatorily coupled to protein synthesis. Secretion is completely inhibited by uncouplers of oxidative phosphorylation and strongly inhibited by the inhibitors of electron transport, cyanide and azide. It is concluded that the transfer of proteins from the cytoplasm to the cell wall is an energy-requiring step.

Full text

PDF
363

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Babad H., Ben-Zvi R., Bdolah A., Schramm M. The mechanism of enzyme secretion by the cell. 4. Effects of inducers, substrates and inhibitors on amylase secretion by rat parotid slices. Eur J Biochem. 1967 Mar;1(1):96–101. doi: 10.1111/j.1432-1033.1967.tb00049.x. [DOI] [PubMed] [Google Scholar]
  2. Bauduin H., Colin M., Dumont J. E. Energy sources for protein synthesis and enzymatic secretion in rat pancreas in vitro. Biochim Biophys Acta. 1969 Feb 18;174(2):722–733. doi: 10.1016/0005-2787(69)90301-3. [DOI] [PubMed] [Google Scholar]
  3. Chrispeels M. J. Biosynthesis of cell wall protein: sequential hydroxylation of proline, glycosylation of hydroxyproline and secretion of the glycoprotein. Biochem Biophys Res Commun. 1970 May 22;39(4):732–737. doi: 10.1016/0006-291x(70)90266-4. [DOI] [PubMed] [Google Scholar]
  4. Chrispeels M. J. Synthesis and Secretion of Hydroxyproline-containing Macromolecules in Carrots: II. In vivo Conversion of Peptidyl Proline to Peptidyl Hydroxyproline. Plant Physiol. 1970 Feb;45(2):223–227. doi: 10.1104/pp.45.2.223. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chrispeels M. J. Synthesis and secretion of hydroxyproline containing macromolecules in carrots. I. Kinetic analysis. Plant Physiol. 1969 Aug;44(8):1187–1193. doi: 10.1104/pp.44.8.1187. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Chrispeels M. J., Varner J. E. Gibberellic Acid-enhanced synthesis and release of alpha-amylase and ribonuclease by isolated barley and aleurone layers. Plant Physiol. 1967 Mar;42(3):398–406. doi: 10.1104/pp.42.3.398. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cleland R. Distribution and metabolism of protein-bound hydroxyproline in an elongating tissue, the Avena coleoptile. Plant Physiol. 1968 Jun;43(6):865–870. doi: 10.1104/pp.43.6.865. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Cleland R., Karlsnes A. M. A possible role of hydroxyproline-containing proteins in the cessation of cell elongation. Plant Physiol. 1967 May;42(5):669–671. doi: 10.1104/pp.42.5.669. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Cole H. A., Wimpenny J. W., Hughes D. E. The ATP pool in Escherichia coli. I. Measurement of the pool using modified luciferase assay. Biochim Biophys Acta. 1967;143(3):445–453. doi: 10.1016/0005-2728(67)90050-3. [DOI] [PubMed] [Google Scholar]
  10. HEYTLER P. G., PRICHARD W. W. A new class of uncoupling agents--carbonyl cyanide phenylhydrazones. Biochem Biophys Res Commun. 1962 May 4;7:272–275. doi: 10.1016/0006-291x(62)90189-4. [DOI] [PubMed] [Google Scholar]
  11. Jamieson J. D., Palade G. E. Intracellular transport of secretory proteins in the pancreatic exocrine cell. 3. Dissociation of intracellular transport from protein synthesis. J Cell Biol. 1968 Dec;39(3):580–588. doi: 10.1083/jcb.39.3.580. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Olson A. C. Proteins and Plant Cell Walls. Proline to Hydroxyproline in Tobacco Suspension Cultures. Plant Physiol. 1964 Jul;39(4):543–550. doi: 10.1104/pp.39.4.543. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Sadava D., Chrispeels M. J. Cell wall protein in plants: autoradiographic evidence. Science. 1969 Jul 18;165(3890):299–300. doi: 10.1126/science.165.3890.299. [DOI] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES