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Plant Physiology logoLink to Plant Physiology
. 1970 Oct;46(4):576–580. doi: 10.1104/pp.46.4.576

Characterization of a Photosynthetic Mutant Strain of Chlamydomonas reinhardi Deficient in Phosphoribulokinase Activity 1

Bernice Moll a, R P Levine a
PMCID: PMC396638  PMID: 16657508

Abstract

A mutant strain of the unicellular green alga, Chlamydomonas reinhardi, is unable to fix carbon dioxide by photosynthesis because it is deficient in phosphoribulokinase activity. The absence of light-dependent carbon dioxide fixation in cells of the mutant strain supports the operation of the Calvin-Benson scheme of photosynthetic carbon dioxide fixation in this organism. No deficiency other than low phosphoribulokinase activity was found which would account for the inability of cells of the mutant strain to fix carbon dioxide by photosynthesis. Activities comparable to those in the wild-type strain were found for eight other enzymes of the Calvin cycle and two enzymes associated with the C4 dicarboxylic acid pathway. The normal rates of nicotinamide adenine dinucleotide phosphate photoreduction and of photosynthetic phosphorylation observed in chloroplast fragments prepared from cells of the mutant strain indicated that the photosynthetic electron transport chain in the mutant is intact.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AXELROD B., JANG R. Purification and properties of phosphoribo-isomerase from alfalfa. J Biol Chem. 1954 Aug;209(2):847–855. [PubMed] [Google Scholar]
  2. Arnon D. I. COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS. Plant Physiol. 1949 Jan;24(1):1–15. doi: 10.1104/pp.24.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BISHOP N. I., GAFFRON H. Photoreduction at lambda 705 millimicrons in adapted algae. Biochem Biophys Res Commun. 1962 Aug 31;8:471–476. doi: 10.1016/0006-291x(62)90299-1. [DOI] [PubMed] [Google Scholar]
  4. Bennoun P., Levine R. P. Detecting mutants that have impaired photosynthesis by their increased level of fluorescence. Plant Physiol. 1967 Sep;42(9):1284–1287. doi: 10.1104/pp.42.9.1284. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chua N. H., Levine R. P. The photosynthetic electron transport chain of Chlamydomonas reinhardi. 8. The 520 nm light-induced absorbance change in the wild-type and mutant strains. Plant Physiol. 1969 Jan;44(1):1–6. doi: 10.1104/pp.44.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. DE LA HABA G., LEDER I. G., RACKER E. Crystalline transketolase from bakers' yeast: isolation and properties. J Biol Chem. 1955 May;214(1):409–426. [PubMed] [Google Scholar]
  7. GINGRAS G., GOLDSBY R. A., CALVIN M. Carbon dioxide metabolism in hydrogen-adapted Scenedesmus. Arch Biochem Biophys. 1963 Feb;100:178–184. [PubMed] [Google Scholar]
  8. Goodenough U. W., Armstrong J. J., Levine R. P. Photosynthetic Properties of ac-31, a Mutant Strain of Chlamydomonas reinhardi Devoid of Chloroplast Membrane Stacking. Plant Physiol. 1969 Jul;44(7):1001–1012. doi: 10.1104/pp.44.7.1001. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Goodenough U. W., Levine R. P. Chloroplast Ultrastructure in Mutant Strains of Chlamydomonas reinhardi Lacking Components of the Photosynthetic Apparatus. Plant Physiol. 1969 Jul;44(7):990–1000. doi: 10.1104/pp.44.7.990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gorman D. S., Levine R. P. Cytochrome f and plastocyanin: their sequence in the photosynthetic electron transport chain of Chlamydomonas reinhardi. Proc Natl Acad Sci U S A. 1965 Dec;54(6):1665–1669. doi: 10.1073/pnas.54.6.1665. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. HORECKER B. L., HURWITZ J., WEISSBACH A. The enzymatic synthesis and properties of ribulose 1,5-diphosphate. J Biol Chem. 1956 Feb;218(2):785–794. [PubMed] [Google Scholar]
  12. HURWITZ J., WEISSBACH A., HORECKER B. L., SMYRNIOTIS P. Z. Spinach phosphoribulokinase. J Biol Chem. 1956 Feb;218(2):769–783. [PubMed] [Google Scholar]
  13. Hatch M. D., Slack C. R. A new enzyme for the interconversion of pyruvate and phosphopyruvate and its role in the C4 dicarboxylic acid pathway of photosynthesis. Biochem J. 1968 Jan;106(1):141–146. doi: 10.1042/bj1060141. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hatch M. D., Slack C. R. NADP-specific malate dehydrogenase and glycerate kinase in leaves and evidence for their location in chloroplasts. Biochem Biophys Res Commun. 1969 Mar 10;34(5):589–593. doi: 10.1016/0006-291x(69)90778-5. [DOI] [PubMed] [Google Scholar]
  15. Hatch M. D., Slack C. R. Photosynthesis by sugar-cane leaves. A new carboxylation reaction and the pathway of sugar formation. Biochem J. 1966 Oct;101(1):103–111. doi: 10.1042/bj1010103. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Hatch M. D., Slack C. R. The participation of phosphoenolpyruvate synthetase in photosynthetic CO2 fixation of tropical grasses. Arch Biochem Biophys. 1967 Apr;120(1):224–225. doi: 10.1016/0003-9861(67)90618-2. [DOI] [PubMed] [Google Scholar]
  17. Johnson H. S., Hatch M. D. The C4-dicarboxylic acid pathway of photosynthesis. Identification of intermediates and products and quantitative evidence for the route of carbon flow. Biochem J. 1969 Aug;114(1):127–134. doi: 10.1042/bj1140127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  19. Levine R. P., Gorman D. S. Photosynthetic electron transport chain of Chlamydomonas reinhardi. 3. Light-induced absorbance changes in chloroplast fragments of the wild type and mutant strains. Plant Physiol. 1966 Oct;41(8):1293–1300. doi: 10.1104/pp.41.8.1293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Levine R. P., Togasaki R. K. A mutant strain of Chlamydomonas reinhardi lacking ribulose diphosphate carboxylase activity. Proc Natl Acad Sci U S A. 1965 May;53(5):987–990. doi: 10.1073/pnas.53.5.987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Loppes R. Damage induced by methyl methanesulfonate (MMS) in Chlamydomonas reinhardi. Z Vererbungsl. 1966;98(3):193–202. doi: 10.1007/BF00888947. [DOI] [PubMed] [Google Scholar]
  22. RACKER E., SCHROEDER E. A. The reductive pentose phosphate cycle. II. Specific C-1 phosphatases for fructose 1,6-diphosphate and sedoheptulose 1,7-diphosphate. Arch Biochem Biophys. 1958 Apr;74(2):326–344. doi: 10.1016/0003-9861(58)90004-3. [DOI] [PubMed] [Google Scholar]
  23. RACKER E. The reductive pentose phosphate cycle. I. Phosphoribulokinase and ribulose diphosphate carboxylase. Arch Biochem Biophys. 1957 Jul;69:300–310. doi: 10.1016/0003-9861(57)90496-4. [DOI] [PubMed] [Google Scholar]
  24. Russell G. K., Gibbs M. Evidence for the participation of the reductive pentose phosphate cycle in photoreduction and the oxyhydrogen reaction. Plant Physiol. 1968 Apr;43(4):649–652. doi: 10.1104/pp.43.4.649. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Russell G. K., Gibbs M. Partial purification and characterization of two fructose diphosphate aldolases from Chlamydomonas mundana. Biochim Biophys Acta. 1967 Jan 11;132(1):145–154. doi: 10.1016/0005-2744(67)90200-8. [DOI] [PubMed] [Google Scholar]
  26. Slack C. R., Hatch M. D. Comparative studies on the activity of carboxylases and other enzymes in relation to the new pathway of photosynthetic carbon dioxide fixation in tropical grasses. Biochem J. 1967 Jun;103(3):660–665. doi: 10.1042/bj1030660. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Sueoka N. MITOTIC REPLICATION OF DEOXYRIBONUCLEIC ACID IN CHLAMYDOMONAS REINHARDI. Proc Natl Acad Sci U S A. 1960 Jan;46(1):83–91. doi: 10.1073/pnas.46.1.83. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. TANNER H. A., BROWN T. E., EYSTER C., TREHARNE R. W. A manganese dependent photosynthetic process. Biochem Biophys Res Commun. 1960 Aug;3:205–210. doi: 10.1016/0006-291x(60)90224-2. [DOI] [PubMed] [Google Scholar]

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