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. 1970 Oct;46(4):614–617. doi: 10.1104/pp.46.4.614

Retardation of Bean Leaf Senescence by Benzyladenine and Its Influence on Phosphate Metabolism 1

N O Adedipe a,2, R A Fletcher a
PMCID: PMC396646  PMID: 16657515

Abstract

The levels of glucose, sugar phosphates, and adenosine phosphates were determined in primary leaves of intact bean plants during normal senescence and compared to leaves in which senescence was delayed by application of benzyladenine (BA). In both cases there was a rise with time in the levels of glucose 1-phosphate, glucose 6-phosphate, and fructose 6-phosphate, and a decline in 2-phosphoglyceric acid, inorganic phosphate, and the adenosine phosphates (AMP, ADP, ATP). The levels of fructose 1,6-diphosphate remained fairly constant. Although the levels of hexose phosphates, adenosine phosphates, and inorganic phosphate were lower in the BA-treated leaves, the incorporation of 32P into these compounds by 3- and 6-week-old plants was higher than in the controls. These results suggest that the retardation of leaf senescence by BA in intact bean plants is associated with increased utilization of metabolites, indicating a more rapid turnover of the adenosine phosphates. It is concluded that this effect is brought about by a regulatory coordination of metabolic processes in relation to energy production and utilization.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allen R. J. The estimation of phosphorus. Biochem J. 1940 Jun;34(6):858–865. doi: 10.1042/bj0340858. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Atkinson D. E. Biological feedback control at the molecular level. Science. 1965 Nov 12;150(3698):851–857. doi: 10.1126/science.150.3698.851. [DOI] [PubMed] [Google Scholar]
  3. Atkinson D. E. The energy charge of the adenylate pool as a regulatory parameter. Interaction with feedback modifiers. Biochemistry. 1968 Nov;7(11):4030–4034. doi: 10.1021/bi00851a033. [DOI] [PubMed] [Google Scholar]
  4. Atkinson D. E., Walton G. M. Adenosine triphosphate conservation in metabolic regulation. Rat liver citrate cleavage enzyme. J Biol Chem. 1967 Jul 10;242(13):3239–3241. [PubMed] [Google Scholar]
  5. Bomsel J. L., Pradet A. Study of adenosine 5'-mono-,di- and triphosphates in plant tissues. IV. Regulation of the level of nucleotides, in vivo, by adenylate kinase: theoretical and experimental study. Biochim Biophys Acta. 1968 Aug 20;162(2):230–242. doi: 10.1016/0005-2728(68)90105-9. [DOI] [PubMed] [Google Scholar]
  6. CRELIN E. S. Mitosis in adult cartilage. Science. 1957 Apr 5;125(3249):650–650. doi: 10.1126/science.125.3249.650. [DOI] [PubMed] [Google Scholar]
  7. Dedolph R. R., Wittwer S. H., Tuli V., Gilbart D. Effect of N-Benzylaminopurine on Respiration & Storage Behavior of Broccoli (Brassica oleracea var. italica). Plant Physiol. 1962 Jul;37(4):509–512. doi: 10.1104/pp.37.4.509. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Geronimo J., Beevers H. Effects of Aging and Temperature on Respiratory Metabolism of Green Leaves. Plant Physiol. 1964 Sep;39(5):786–793. doi: 10.1104/pp.39.5.786. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Halevy A. H., Dilley D. R., Wittwer S. H. Senescence inhibition and respiration induced by growth retardants and N-benzyladenine. Plant Physiol. 1966 Sep;41(7):1085–1089. doi: 10.1104/pp.41.7.1085. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Rowan K. S. Phosphorus metabolism in plants. Int Rev Cytol. 1966;19:301–390. doi: 10.1016/s0074-7696(08)60571-9. [DOI] [PubMed] [Google Scholar]
  11. Scott R. W., Moore W. E., Effland M. J., Millett M. A. Ultraviolet spectrophotometric determination of hexoses, pentoses, and uronic acids after their reactions with concentrated sulfuric acid. Anal Biochem. 1967 Oct;21(1):68–80. doi: 10.1016/0003-2697(67)90084-x. [DOI] [PubMed] [Google Scholar]
  12. TULI V., DILLEY D. R., WITTWER S. H. N6-BENZYLADENINE: INHIBITOR OF RESPIRATORY KINASES. Science. 1964 Dec 11;146(3650):1477–1479. doi: 10.1126/science.146.3650.1477. [DOI] [PubMed] [Google Scholar]
  13. Weinstein L. H., McCune D. C., Mancini J. F., van Leuken P. Acid-soluble nucleotides of pinto bean leaves at different stages of development. Plant Physiol. 1969 Nov;44(11):1499–1510. doi: 10.1104/pp.44.11.1499. [DOI] [PMC free article] [PubMed] [Google Scholar]

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