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. 1970 Nov;46(5):666–673. doi: 10.1104/pp.46.5.666

Compartments and Fluxes of K+, NA+, and CL− in Avena Coleoptile Cells 1

Wayne S Pierce a, N Higinbotham a
PMCID: PMC396658  PMID: 16657527

Abstract

By the compartmental analysis method of MacRobbie and Dainty, and Pitman, estimates of K+, Na+, and Cl− concentrations and fluxes were obtained for the cytoplasm and vacuole of coleoptile cells of oat, Avena sativa L. cv. Victory. Double labeling was used in experiments with 42K plus 22Na and with 42K plus 36Cl in a complete nutrient solution. At the plasmalemma, according to the Ussing-Teorell flux ratio equation, Na+ is pumped out and Cl− is actively transported inward. The results with K+ are less conclusive, but it is probably pumped in. At the tonoplast there is an active inward transport of Na+ and probably of K+, but the status of Cl− is uncertain, depending upon whether there is an electrical potential difference between the cytoplasm and vacuole. The results suggest that ion selectivity resides mostly in the plasmalemma. Possible errors in the estimates and interpretations are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Coster H. G., George E. P. A thermodynamic analysis of fluxes and flux-ratios in bioligical membranes. Biophys J. 1968 Apr;8(4):457–469. doi: 10.1016/s0006-3495(68)86500-2. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cram W. J. Compartmentation and exchange of chloride in carrot root tissue. Biochim Biophys Acta. 1968 Nov 5;163(3):339–353. doi: 10.1016/0005-2736(68)90119-3. [DOI] [PubMed] [Google Scholar]
  3. DIAMOND J. M., SOLOMON A. K. Intracellular potassium compartments in Nitella axillaris. J Gen Physiol. 1959 May 20;42(5):1105–1121. doi: 10.1085/jgp.42.5.1105. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Davis R. F., Higinbotham N. Effects of external cations and respiratory inhibitors on electrical potential of the xylem exudate of excised corn roots. Plant Physiol. 1969 Oct;44(10):1383–1392. doi: 10.1104/pp.44.10.1383. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. ETHERTON B., HIGINBOTHAM N. Transmembrane potential measurements of cells of higher plants as related to salt uptake. Science. 1960 Feb 12;131(3398):409–410. doi: 10.1126/science.131.3398.409. [DOI] [PubMed] [Google Scholar]
  6. Etherton B. Steady State Sodium and Rubidium Effluxes in Pisum sativum Roots. Plant Physiol. 1967 May;42(5):685–690. doi: 10.1104/pp.42.5.685. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Etherton B. Vacuolar and Cytoplasmic Potassium Concentrations in Pea Roots in Relation to Cell-to-Medium Electrical Potentials. Plant Physiol. 1968 May;43(5):838–840. doi: 10.1104/pp.43.5.838. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Higinbotham N., Etherton B., Foster R. J. Mineral ion contents and cell transmembrane electropotentials of pea and oat seedling tissue. Plant Physiol. 1967 Jan;42(1):37–46. doi: 10.1104/pp.42.1.37. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Macklon A. E., Higinbotham N. Active and passive transport of potassium in cells of excised pea epicotyls. Plant Physiol. 1970 Feb;45(2):133–138. doi: 10.1104/pp.45.2.133. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Macklon A. E., Higinbotham N. Electropotential in excised pea epicotyls. Plant Physiol. 1968 Jun;43(6):888–892. doi: 10.1104/pp.43.6.888. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Pitman M. G., Saddler H. D. Active sodium and potassium transport in cells of barley roots. Proc Natl Acad Sci U S A. 1967 Jan;57(1):44–49. doi: 10.1073/pnas.57.1.44. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Poole R. J. The influence of the intracellular potential on potassium uptake by beetroot tissue. J Gen Physiol. 1966 Jan;49(3):551–563. doi: 10.1085/jgp.49.3.551. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Vaughan B. E., Evans E. C., Hutchin M. E. Polar transport characteristics of radiostrontium and radiocalcium in isolated corn root segments. Plant Physiol. 1967 May;42(5):747–750. doi: 10.1104/pp.42.5.747. [DOI] [PMC free article] [PubMed] [Google Scholar]

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