Skip to main content
Plant Physiology logoLink to Plant Physiology
. 1970 Dec;46(6):831–838. doi: 10.1104/pp.46.6.831

Synthesis and Transport of Hydroxyproline-rich Components in Suspension Cultures of Sycamore-Maple Cells 1

W V Dashek a,2
PMCID: PMC396692  PMID: 16657552

Abstract

Plant cell walls contain a glycoprotein rich in hydroxyproline. To determine how Acer pseudoplatanus L. cells transport this glycoprotein to the wall, the pulse-chase technique was used to follow changes in specific radio-activity of hydroxyproline and proline in isolated, mitochondrial, Golgi, microsomal, soluble protein, and wall fractions. The turnover rates or changes in specific radioactivity of cytoplasmic hydroxyproline in these cell fractions indicated that the bulk of this hydroxyproline was transferred not by the Golgi apparatus but by a smooth membranous component.

Full text

PDF
831

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CHINARD F. P. Photometric estimation of proline and ornithine. J Biol Chem. 1952 Nov;199(1):91–95. [PubMed] [Google Scholar]
  2. Chrispeels M. J. Synthesis and secretion of hydroxyproline containing macromolecules in carrots. I. Kinetic analysis. Plant Physiol. 1969 Aug;44(8):1187–1193. doi: 10.1104/pp.44.8.1187. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cook G. M., Laico M. T., Eylar E. H. Biosynthesis of glycoproteins of the Ehrlich ascites carcinoma cell membranes. Proc Natl Acad Sci U S A. 1965 Jul;54(1):247–252. doi: 10.1073/pnas.54.1.247. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dashek W. V., Rosen W. G. Electron microscopical localization of chemical components in the growth zone of lily pollen tubes. Protoplasma. 1966;61(1):192–204. doi: 10.1007/BF01247919. [DOI] [PubMed] [Google Scholar]
  5. Holleman J. M., Key J. L. Inactive and protein precursor pools of amino acids in the soybean hypocotyl. Plant Physiol. 1967 Jan;42(1):29–36. doi: 10.1104/pp.42.1.29. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Holleman J. Direct incorporation of hydroxyproline into protein of sycamore cells incubated at growth-inhibitory levels of hydroxyproline. Proc Natl Acad Sci U S A. 1967 Jan;57(1):50–54. doi: 10.1073/pnas.57.1.50. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. LAMPORT D. T. CELL SUSPENSION CULTURES OF HIGHER PLANTS: ISOLATION AND GROWTH ENERGETICS. Exp Cell Res. 1964 Jan;33:195–206. doi: 10.1016/s0014-4827(64)81026-0. [DOI] [PubMed] [Google Scholar]
  8. Lamport D. T. The isolation and partial characterization of hydroxyproline-rich glycopeptides obtained by enzymic degradation of primary cell walls. Biochemistry. 1969 Mar;8(3):1155–1163. doi: 10.1021/bi00831a049. [DOI] [PubMed] [Google Scholar]
  9. MOLLENHAUER H. H., WHALEY W. G., LEECH J. H. A function of the Golgi apparatus in outer rootcap cells. J Ultrastruct Res. 1961 Apr;5:193–200. doi: 10.1016/s0022-5320(61)90014-4. [DOI] [PubMed] [Google Scholar]
  10. MORRE D. J., MOLLENHAUER H. H., CHAMBERS J. E. GLUTARALDEHYDE STABILIZATION AS AN AID TO GOLGI APPARATUS ISOLATION. Exp Cell Res. 1965 Jun;38:672–675. doi: 10.1016/0014-4827(65)90392-7. [DOI] [PubMed] [Google Scholar]
  11. MORRE D. J., MOLLENHAUER H. H. ISOLATION OF THE GOLGI APPARATUS FROM PLANT CELLS. J Cell Biol. 1964 Nov;23:295–305. doi: 10.1083/jcb.23.2.295. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Olson A. C. Proteins and Plant Cell Walls. Proline to Hydroxyproline in Tobacco Suspension Cultures. Plant Physiol. 1964 Jul;39(4):543–550. doi: 10.1104/pp.39.4.543. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. REYNOLDS E. S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963 Apr;17:208–212. doi: 10.1083/jcb.17.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Ray P. M., Shininger T. L., Ray M. M. ISOLATION OF beta-GLUCAN SYNTHETASE PARTICLES FROM PLANT CELLS AND IDENTIFICATION WITH GOLGI MEMBRANES. Proc Natl Acad Sci U S A. 1969 Oct;64(2):605–612. doi: 10.1073/pnas.64.2.605. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Rosen W. G., Gawlik S. R. Fine structure of lily pollen tubes following various fixation and staining procedures. Protoplasma. 1966;61(1):181–191. doi: 10.1007/BF01247918. [DOI] [PubMed] [Google Scholar]
  16. SABATINI D. D., BENSCH K., BARRNETT R. J. Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation. J Cell Biol. 1963 Apr;17:19–58. doi: 10.1083/jcb.17.1.19. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Siegel M. R., Sisler H. D. Site of action of cycloheximide in cells of Saccharomyces pastorianus. 3. Further studies on the mechanism of action and the mechanism of resistance in saccharomyces species. Biochim Biophys Acta. 1965 Aug 10;103(4):558–567. [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES