Abstract
In the biosynthesis of fatty acids from 1-14C-acetate by intact spinach chloroplasts, ATP and Triton X-100 exert opposing effects on the conversion of palmitic acid to stearic acid; thus, ATP decreases the conversion and Triton X-100 increases the conversion. Changes in the availability of photosynthetically generated reduced nicotinamide adenine dinucleotide phosphate apparently does not markedly affect the C16-C18 ratio. Various H2O2-generating systems, such as viologen dyes, inhibit oleate synthesis from acetate and cause stearate to accumulate. Catalase partially reverses the effect of these days.
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Selected References
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- Brooks J. L., Stumpf P. K. Fat metabolism in higher plants. XXXIX. Properties of a soluble fatty acid synthesizing system from lettuce chloroplasts. Arch Biochem Biophys. 1966 Sep 26;116(1):108–116. doi: 10.1016/0003-9861(66)90019-1. [DOI] [PubMed] [Google Scholar]
- Cockburn W., Baldry C. W., Walker D. A. Some effects of inorganic phosphate on O2 evolution by isolated chloroplasts. Biochim Biophys Acta. 1967;143(3):614–624. doi: 10.1016/0005-2728(67)90067-9. [DOI] [PubMed] [Google Scholar]
- Givan C. V., Givan A. L., Leech R. M. Photoreduction of alpha-Ketoglutarate to Glutamate by Vicia faba Chloroplasts. Plant Physiol. 1970 May;45(5):624–630. doi: 10.1104/pp.45.5.624. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HAVIR E. A., GIBBS M. STUDIES ON THE REDUCTIVE PENTOSE PHOSPHATE CYCLE IN INTACT AND RECONSTITUTED CHLOROPLAST SYSTEMS. J Biol Chem. 1963 Oct;238:3183–3187. [PubMed] [Google Scholar]
- Heber U. W., Santarius K. A. Compartmentation and reduction of pyridine nucleotides in relation to photosynthesis. Biochim Biophys Acta. 1965 Nov 29;109(2):390–408. doi: 10.1016/0926-6585(65)90166-4. [DOI] [PubMed] [Google Scholar]
- Izawa S., Good N. E. The number of sites sensitive to 3-(3,4-dichlorophenyl)-1,1-dimethylurea,3-(4-chlorophenyl)-1,1-dimethylurea and 2-chloro-4-(2-propylamino)-6-ethylamino-s-triazine in isolated chloroplasts. Biochim Biophys Acta. 1965 May 25;102(1):20–38. doi: 10.1016/0926-6585(65)90200-1. [DOI] [PubMed] [Google Scholar]
- Jensen R. G., Bassham J. A. Photosynthesis by isolated chloroplasts. Proc Natl Acad Sci U S A. 1966 Oct;56(4):1095–1101. doi: 10.1073/pnas.56.4.1095. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kok B., Rurainski H. J., Owens O. V. The reducing power generated in photoact I of photosynthesis. Biochim Biophys Acta. 1965 Nov 29;109(2):347–356. doi: 10.1016/0926-6585(65)90162-7. [DOI] [PubMed] [Google Scholar]
- MUDD J. B., McMANUS T. T. Metabolism of acetate by cellfree preparations from spinach leaves. J Biol Chem. 1962 Jul;237:2057–2063. [PubMed] [Google Scholar]
- Neumann J., Jagendorf A. Uncoupling photophosphorylation by detergents. Biochim Biophys Acta. 1965 Nov 29;109(2):382–389. doi: 10.1016/0926-6585(65)90165-2. [DOI] [PubMed] [Google Scholar]
- STUMPF P. K., JAMES A. T. The biosynthesis of long-chain fatty acids by lettuce chloroplast preparations. Biochim Biophys Acta. 1963 Feb 19;70:20–32. doi: 10.1016/0006-3002(63)90715-7. [DOI] [PubMed] [Google Scholar]
- VAGELOS P. R. LIPID METABOLISM. Annu Rev Biochem. 1964;33:139–172. doi: 10.1146/annurev.bi.33.070164.001035. [DOI] [PubMed] [Google Scholar]
- VERNON L. P., ZAUGG W. S. Photoreductions by fresh and aged chloropasts: requirement for ascorbate and 2, 6-dichlorophenolindophenol with aged chloroplasts. J Biol Chem. 1960 Sep;235:2728–2733. [PubMed] [Google Scholar]
- Zweig G., Shavit N., Avron M. Diquat (I,I'-ethylene-2,2'-dipyridylium dibromide) in photo-reactions of isolated chloroplasts. Biochim Biophys Acta. 1965 Nov 29;109(2):332–346. [PubMed] [Google Scholar]