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. 1971 Jul;48(1):5–8. doi: 10.1104/pp.48.1.5

On the Mechanism and Stoichiometry of the Oxidation of Hydrazine by Illuminated Chloroplasts 1

K E Mantai a,2, G Hind a
PMCID: PMC396789  PMID: 16657732

Abstract

Hydrazine can support a rapid oxygen uptake in illuminated chloroplasts. The oxygen uptake rate is inhibited by 3-(3,4-dichlorophenyl)-1, 1-dimethylurea but is only slightly increased by added methyl viologen, and little H2O2 is produced. The pH optimum for hydrazine-dependent oxygen uptake is much higher than that of the Hill reaction. Addition of Mn (II) increases the rate of oxygen uptake in the light and causes the reaction to continue in the dark, the dark rate being dependent on the duration of the preceding light period. Flash yield experiments show that at least six electrons are transferred from hydrazine per flash compared to one electron per flash when water is the electron donor.

We conclude that most of the oxygen uptake in the presence of hydrazine is due to a direct oxidation of the hydrazine by oxygen which is catalyzed by some factor, possibly Mn (III), produced by chloroplasts in the light. Other artificial electron donors are shown to support such artefactual oxygen uptake to varying extents.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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