Abstract
Enzymatically separated tobacco leaf cells took up amino acids, uracil, and uridine from the incubation medium and incorporated them into proteins and RNA, respectively, at a linear rate for approximately 30 hours. Both uptake and incorporation were light-dependent, although cells prepared from preilluminated plants or preillumination of cells allowed some uptake and incorporation to occur in the dark. The light was necessary to satisfy a photosynthetic requirement, but could be replaced in part by ATP in the medium.
Several lines of evidence support the conclusion that the rate of uptake of amino acids, uracil, and uridine was dependent upon the subsequent incorporation of these compounds into macromolecules.
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Selected References
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- Jensen R. G., Francki R. I., Zaitlin M. Metabolism of separated leaf cells: I. Preparation of photosynthetically active cells from tobacco. Plant Physiol. 1971 Jul;48(1):9–13. doi: 10.1104/pp.48.1.9. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kirk J. T. Failure to detect effects of cycloheximide on energy metabolism in Euglena gracilis. Nature. 1970 Apr 11;226(5241):182–182. doi: 10.1038/226182a0. [DOI] [PubMed] [Google Scholar]
- Leake C. D., Rosenbaum E. E., Jacob S. W. Summary of the New York Academy of Sciences symposium on the "biological actions of dimethyl sulfoxide". Ann N Y Acad Sci. 1967 Mar 15;141(1):670–671. doi: 10.1111/j.1749-6632.1967.tb34939.x. [DOI] [PubMed] [Google Scholar]
- MACROBBIE E. A. THE NATURE OF THE COUPLING BETWEEN LIGHT ENERGY AND ACTIVE ION TRANSPORT IN NITELLA TRANSLUCENS. Biochim Biophys Acta. 1965 Jan 25;94:64–73. doi: 10.1016/0926-6585(65)90008-7. [DOI] [PubMed] [Google Scholar]