Abstract
We report here on the genome sequence of Yersinia similis 228T isolated in Germany. The genome has a size of 4.9 Mb and a G+C content of 47% and is predicted to contain 4,135 coding sequences. Annotation of the 60,687-bp extrachromosomal element predicted 67 coding sequences and a G+C content of 47.8%.
GENOME ANNOUNCEMENT
Initially typed as Yersinia pseudotuberculosis, Yersinia similis was identified as a separate species on the basis of differences in DNA-DNA relatedness, the presence of a specific 16S rRNA gene sequence, and the inability to ferment melibiose (1). Y. similis expresses the Y. pseudotuberculosis-derived mitogen YPMb but lacks the virulence plasmid pYV and the high-pathogenicity island and is therefore believed to be apathogenic for humans (1, 2). To date, Y. similis strains have only been isolated from small mammals and the environment. Y. similis isolates may cause diagnostic problems, as they cannot be biochemically distinguished from Y. pseudotuberculosis by commercial test kits, such as the widely used API 20E (1, 2). Y. similis Y228T serovar VI was isolated from a rabbit in Germany.
This paper announces the genome sequence of Y. similis Y228T. Genomic DNA was isolated from an overnight culture grown in LB medium with 1% glucose with a Qiagen Genomic-tip 100/Q and genomic DNA buffer set (Qiagen, Germany). DNA quality was examined by using both a NanoDrop spectrophotometer (Thermo Scientific, Germany) and a Qubit 2.0 fluorometer (Life Technologies, Germany). Whole-genome sequencing was performed on a Pacific Biosciences RS sequencer with SMRT Technology PacBio RS II (Pacific Biosciences, Menlo Park, CA) at GATC Biotech (Germany) using standard protocols according to the manufacturer’s instructions, which were followed throughout the sequencing process. De novo assembly was performed with SMRT portal version 2.1.0 (Daemon version 2.1.0, SMRT View version 2.1, SMRTpipe version 2.1.0; Pacific Biosciences) from five contigs, with an N50 contig length of 4,779,767 bp. The genome has a size of 4,903,722 bp and a G+C content of 47%. Genome annotation was done using the NCBI Prokaryotic Genome Annotation Pipeline (http://www.ncbi.nlm.nih.gov/genome/annotation_prok/) and predicted 4,356 genes, 111 pseudogenes, 4,135 coding sequences (CDSs), and 86 tRNA and 22 rRNA genes. By using BLASTn 2.2.29 (3), a 60,687-bp extrachromosomal element with a G+C content of 47.8% was aligned to pGTD4 of Y. pseudotuberculosis (4), demonstrating the presence of a plasmid; its annotation predicted 67 CDSs.
The availability of the sequence of Y. similis 228T may help to enlighten the evolution of apathogenic Yersinia to highly pathogenic Yersinia within the Y. pseudotuberculosis complex.
Nucleotide sequence accession numbers.
The sequences of Y. similis 228T and the putative plasmid of Y. similis 228T have been deposited at DDBJ/EMBL/GenBank under the accession no. CP007230 and CP007231, respectively.
ACKNOWLEDGMENTS
This work was funded by the European Union, Anticipating the global onset of novel epidemics (Antigone FP7-health), project 278976.
We are very grateful for the help provided by C. König (Pacific Biosciences).
Footnotes
Citation Sprague LD, Neubauer H. 2014. Genome sequence of Yersinia similis Y228T, a member of the Yersinia pseudotuberculosis complex. Genome Announc. 2(2):e00216-14. doi:10.1128/genomeA.00216-14.
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