Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1971 Dec;48(6):671–674. doi: 10.1104/pp.48.6.671

Rapid Development of Mitochondria in Pea Cotyledons during the Early Stage of Germination

Yoshihiko Nawa a, Tadashi Asahi a
PMCID: PMC396927  PMID: 16657859

Abstract

Rapid increases in activities and components of mitochondrial particles isolated from cotyledons of Pisum sativum var. Alaska during the early stage of germination are described. Respiratory rate of the cotyledons increased rapidly as hydration proceeded. A similar but slightly delayed increase in respiratory activity of the isolated mitochondrial fraction was observed. The respiratory control ratio and adenosine 5′-pyrophosphate/oxygen ratio rose during imbibition. Cytochrome oxidase and malate dehydrogenase activities in the mitochondrial fraction increased during the initial phase of imbibition. The increase seemed to precede that in respiratory activity. A significant activity of cytochrome oxidase and most of the malate dehydrogenase activity in the cotyledons were present in the postmitochondrial fraction in the case of the dry seeds. Mitochondrial protein and phospholipid also increased during imbibition, and the rise in the components seemed to concur with that in respiratory activity. The mechanism of mitochondrial development during imbibition is discussed.

Full text

PDF
671

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Breidenbach R. W., Castelfranco P., Criddle R. S. Biogenesis of Mitochondria in Germinating Peanut Cotyledons II. Changes in Cytochromes and Mitochondrial DNA. Plant Physiol. 1967 Aug;42(8):1035–1041. doi: 10.1104/pp.42.8.1035. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Breidenbach R. W., Castelfranco P., Peterson C. Biogenesis of mitochondria in germinating peanut cotyledons. Plant Physiol. 1966 May;41(5):803–809. doi: 10.1104/pp.41.5.803. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CHANCE B., WILLIAMS G. R. A simple and rapid assay of oxidative phosphorylation. Nature. 1955 Jun 25;175(4469):1120–1121. doi: 10.1038/1751120a0. [DOI] [PubMed] [Google Scholar]
  4. CHANCE B., WILLIAMS G. R. The respiratory chain and oxidative phosphorylation. Adv Enzymol Relat Subj Biochem. 1956;17:65–134. doi: 10.1002/9780470122624.ch2. [DOI] [PubMed] [Google Scholar]
  5. Cherry J. H. Nucleic Acid, Mitochondria, & Enzyme Changes in Cotyledons of Peanut Seeds during Germination. Plant Physiol. 1963 Jul;38(4):440–446. doi: 10.1104/pp.38.4.440. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. FOLCH J., LEES M., SLOANE STANLEY G. H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed] [Google Scholar]
  7. HAGIHARA B. Techniques for the application of polarography to mitochondrial respiration. Biochim Biophys Acta. 1961 Jan 1;46:134–142. doi: 10.1016/0006-3002(61)90656-4. [DOI] [PubMed] [Google Scholar]
  8. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  9. Young J. L., Huang R. C., Vanecko S., Marks J. D., Varner J. E. Conditions Affecting Enzyme Synthesis in Cotyledons of Germinating Seeds. Plant Physiol. 1960 May;35(3):288–292. doi: 10.1104/pp.35.3.288. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES